20.500.12556/RUL-185525 Pomegranate juice treatment prevents carbon tetrachloride(CCl4)-induced testicular damage in rats a biochemical and histological study Zdravljenje s sokom granatnega jabolka preprečuje poškodbe mod zaradi ogljikovega tetraklorida (CCl4) pri podganah: biokemična in histološka študija We investigated the side effects of carbon tetrachloride (CCl4) on testicular tissue and explored the protective effects of pomegranate juice (PJ) against CCl4 expo-sure. Twenty-eight male Wistar albino rats were randomly divided into four groups as follows: Control, PJ, CCl4, and CCl4+PJ. We assessed serum testosterone levels and evaluated carboxylesterase (CaE), malondialdehyde (MDA), glutathione (GSH), glutathi-one reductase (GR) and glutathione Stransferase (GST) parameters. Histopathological changes were examined using CD68 immunoreactivity to detect macrophage activity. Analysis revealed a significant increase in CaE activity in the PJ group that compared to the control group (p < 0.05). CCl4 exposure reduced CaE activity, which was partially restored by PJ in the CCl4+PJ group (p < 0.05). PJ also significantly reduced the elevated MDA levels induced by CCl4 (p < 0.05). CCl4 alone decreased the reduced glutathione (GSH) levels (p < 0.05), however PJ had no effect on GSH levels (p > 0.05). Neither CCl4 nor PJ had an effect on glutathione reductase (GR) and glutathione S-transferase (GST) activity (p > 0.05). In conclusion, PJ reversed histological damage and restored reduced testosterone levels. Furthermore, it alleviated CCl4-induced oxidative stress by utilizing an alternative antioxidant system rather than glutathione. CCl4 toxicity infertility pomegranate juice carboxylesterase oxidative stress histopathology true false true Angleški jezik Slovenski jezik Članek v reviji 2026-08-07 09:41:49 2026-08-07 09:41:53 2026-09-10 10:38:09 0000-00-00 00:00:00 2025 0 0 Str. 83-93 iss. 2 Vol. 62 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.09:616 2385-8761 285374467 PomegranateRUL.pdf PomegranateRUL.pdf 1 6C876DD818E4FA6DE8AC88C1DB7E0BAF 4c32e4bf055f6dfb9feaab458d148946182339f5784b18277210f38363b306d4 274c5bd0-9233-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=244094 https://www.slovetres.si/index.php/SVR/article/view/2056/892 1 1e123312-9233-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=244093 Veterinarska fakulteta Slovenian veterinary research 0 0 0