20.500.12556/RUL-185486 Tea tree (Melaleuca alternifolia) and geranium (Pelargonium graveolens) essential oils as new anesthetics in rainbow trout (Oncorhynchus mykiss) Imunohistokemične in histomorfometrične spremembe tankega črevesa, povezane z dodatkom natrijevega butirata pri piščancih In the present study was investigated anesthetic and histopathological effects of tea tree (Melaleuca alternifolia) and geranium (Pelargonium graveolens) essential oils in rainbow trout. These essential oils were applied at 20, 50, 70, 100, 150, 200, 300, 400, 500 and 600 mg L-1 concentrations to determine the anesthetic effect. As a result of this study, major components were found as beta-citronellol (27.52%) for geranium oil and 4-terpineol (40.77%) for tea tree oil. The lowest effective doses of geranium and tea tree oils were 400 mg L-1 and 300 mg L-1 for deep anesthesia of fish, respectively. At these doses, anesthesia induction and recovery times were 129.00 and 247.00 s for geranium essential oil, 118.67 and 65s for tea tree essential oil, respectively. No histopathological findings in liver, kidney and gill tissues were observed in anesthetized fish for each essential oil. In conclusion, geranium and tea tree oils can use as safe and effective anesthetics for rainbow trout. Študija je preučevala anestetične in histopatološke učinke eteričnih olj čajevca (Melaleuca alternifolia) in pelargonije (Pelargonium graveolens) na šarenko. Ti eterični olji sta bili uporabljeni v koncentracijah 20, 50, 70, 100, 150, 200, 300, 400, 500 in 600 mg L-1 za določitev anestetičnega učinka. Na podlagi te študije so bile ugotovljene naslednje glavne sestavine: beta-citronelol (27,52 %) za olje geranije in 4-terpineol (40,77 %) za olje čajevca. Najnižji učinkoviti odmerki olja geranije in čajevca so bili 400 mg L-1 oziroma 300 mg L-1 za globoko anestezijo rib. Pri teh odmerkih sta bila čas indukcije anestezije in čas prebujanja iz anestezije 129,00 in 247,00 s za eterično olje geranije ter 118,67 in 65 s za eterično olje čajevca. Pri anesteziranih ribah pri nobenem eteričnem olju niso opazili histopatoloških sprememb v tkivih jeter, ledvic in škrg. Iz tega sledi, da se olji geranije in čajevca lahko uporabljata kot varna in učinkovita anestetika za šarenko. Oncorhynchus mykiss anesthesia histopathology tea tree geranium Oncorhynchus mykiss anestezija histopatologija čajevec pelargonija true false true Angleški jezik Slovenski jezik Članek v reviji 2026-08-06 14:18:48 2026-08-06 14:18:52 2026-08-07 04:08:25 0000-00-00 00:00:00 2025 0 0 Str. 223-231 iss. 3 Vol. 62 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-01-01 665.52:612.887:639.21 2385-8761 10.26873/SVR-1809-2023 286362627 tea.pdf tea.pdf 1 BA5807B7949AE044E64E1C4F550A4A01 496ffeef33debb30f92640cd19ec5b261d04d0daa87a64b725fe9444f666440d cd8d5ced-9190-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=244029 https://www.slovetres.si/index.php/SVR/article/view/1809/929 1 a48df57c-9190-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=244026 Veterinarska fakulteta Slovenian veterinary research 0 0 0