20.500.12556/RUL-185464 Multiscale imaging of Pleurotus ostreatus fruiting bodies – from 3D anatomy to the ultrastructure Slikovna aAnaliza trosnjakov glive Pleurotus ostreatus – od 3D anatomije do ultrastrukture Oyster mushroom, Pleurotus ostreatus, is economically important and widely used as a model organism, yet a comprehensive characterisation of its fruiting body structure across multiple levels of biological organisation is lacking. We applied a multiscale imaging, combining micro-computed tomography (micro-CT), light microscopy of paraffin, cryostat and semithin sections, and transmission electron microscopy to investigate the structure of P. ostreatus young fruiting bodies, and we provide a direct comparison of different sample preparation procedures. Micro-CT enabled 3D anatomy visualisation, revealing distinct tissue regions: a loose trama in the stipe and pileus medulla, a dense peripheral layer of pileus and gills, and a thin, loose layer covering pileus and gills. Histological analyses provided detailed information on tissue organisation, complementing the micro-CT images, including hyphal arrangement, gill architecture, hymenial layer, and basidia. Cryosections preserved overall tissue morphology and proved suitable for future molecular localisation studies. Transmission electron microscopy of Agar 100 and LR White embedded samples resolved the ultrastructure of hyphae and basidia, revealing nuclei, mitochondria, endoplasmic reticulum, vacuoles, cell walls, and dolipore septa with perforated pore cap. The study demonstrates the value of integrating imaging techniques to characterise the mushroom structure across scales and provides a toolbox for future investigations of fruiting bodies. Bukov ostrigar (Pleurotus ostreatus) je gospodarsko pomembna goba in pogosto uporabljen kot modelni organizem. Kljub temu je morfologija trosnjaka ostrigarja slabo opredeljena in podrobnih raziskav strukture na več ravneh biološke organizacije ni. V raziskavi smo uporabili kombinacijo slikovnih tehnik, in sicer mikro-računalniško tomografijo (mikro-CT), svetlobno mikroskopijo parafinskih, kriostatskih in poltankih rezin ter presevno elektronsko mikroskopijo, za analizo strukture mladega trosnjaka vrste P. ostreatus. Poleg tega raziskava vključuje neposredno primerjavo različnih postopkov priprave vzorcev. Tehnika mikro-CT je omogočila tridimenzionalno vizualizacijo anatomije trosnjakov in prikazala različna tkivna območja: rahlo tkivo v betu in sredici klobuka, gosta robna plast klobuka in lističev ter tanko rahlo plast, ki prekriva klobuk in lističe. S histološko analizo smo podrobno pokazali organizacijo tkiv, kar dopolnjuje rezultate iz mikro-CT analiz, vključno z razporeditvijo hif, arhitekturo lističev, trosovnice in bazidijev. Kriostatske rezine so podale splošno morfologijo tkiva, primerno za nadaljnje študije molekularne lokalizacije. S presevno elektronsko mikroskopijo vzorcev vklopljenih v smolo Agar 100 in v smolo LR White smo pokazali ultrastrukturo hif in bazidijev, vidna so bila jedra, mitohondriji, endoplazemski retikulum, vakuole, celične stene in doliporne septe s perforirano porno kapico. Raziskava kaže pomen integracije slikovnih tehnik, s katerimi lahko opredelimo strukturo gobe na različnih prostorskih ravneh, in zagotavlja orodje za prihodnje raziskave trosnjakov. micro-CT microscopy fungi hyphae cell wall septal pore mikro-CT mikroskopija glive hife celična stena septalna pora true false true Angleški jezik Slovenski jezik Članek v reviji 2026-08-06 08:46:50 2026-08-06 08:46:51 2026-08-07 04:08:22 0000-00-00 00:00:00 2026 0 0 Str. 64-86 no. 3 Vol. 69 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 543.456 1854-3073 10.14720/abs.69.3.28176 286891523 RAZ_Mrak_Polona_2026.pdf RAZ_Mrak_Polona_2026.pdf 1 36EAB399B5806A57CFBFFFDF879218AD 748c047d91f75c32d3218cd38632b07666576828803123a950a0def3fa7b104a 7d9a734e-9162-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=243979 https://journals.uni-lj.si/abs/article/view/28176 0 88c53e6e-9162-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=243980 Biotehniška fakulteta 0 0 0