20.500.12556/RUL-185257 Identification and prioritisation of tumour antigen candidates from 79 glioblastoma transcriptomes Glioblastoma (GBM) is an aggressive brain tumour with limited responsiveness to current immunotherapeutic approaches, partly due to its low mutational burden and intra-tumour heterogeneity. A systematic understanding of the tumour antigen landscape is therefore essential for advancing tumour immunology and supporting rational development of immunotherapeutic strategies. In this study, we performed whole-transcriptome sequencing of RNA extracted from 79 formalin-fixed paraffin-embedded (FFPE) IDH-wildtype GBM samples to systematically identify and prioritise candidate tumour antigens derived from three sources: single-nucleotide variants (SNVs), overexpressed tumour-associated antigens (TAAs), and gene fusion events. Candidate peptides were evaluated using integrated computational criteria, including transcript expression, predicted antigen processing features, peptide-HLA binding affinity and stability. Across the cohort, mutation-derived tumor-specific antigens (TSAs) were largely private to individual samples, whereas TAAs constituted a larger and more recurrent candidate pool. Despite comparable predicted binding characteristics across antigen classes, recurrence patterns differed substantially, reflecting their distinct biological origins. Fusion-derived candidates were rare and sample-specific. Predicted peptide presentation was disproportionately associated with a limited subset of HLA class I alleles. Collectively, this study provides a systematically prioritized catalogue of transcriptionally expressed GBM antigen candidates and offers a comparative evaluation of mutation-, expression-, and fusion-derived antigen sources within a unified transcriptome-based framework. computational immunology glioblastoma HLA class I transcriptome sequencing tumor antigens računalniška imunologija glioblastom HLA razred I sekvenciranje transkriptomov tumorski antigeni true false true Angleški jezik Slovenski jezik Članek v reviji 2026-07-30 08:36:35 2026-07-30 08:36:40 2026-10-06 16:39:27 0000-00-00 00:00:00 2026 0 0 13 str. iss. 5, art. 149 Vol. 75 Apr. 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616 0340-7004 10.1007/s00262-026-04390-3 276023555 RAZ_Kert_Spela_2026.pdf RAZ_Kert_Spela_2026.pdf 1 C8ADE22F26BD669FC9DDEBA1235EEBE0 e0dc6fd5bf86013f287482d28df4743231354b5f628415dc14432f80c2908659 67a40db0-8be1-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=243599 https://link.springer.com/article/10.1007/s00262-026-04390-3 1 c0e31129-8be0-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=243596 Medicinska fakulteta 0 0 0