20.500.12556/RUL-185022 Biomolecular characterization of human deep fascia in type 2 diabetes by FTIR spectroscopy and integrated chemometric analysis Background Type 2 diabetes mellitus (T2DM) is associated with adverse changes in connective tissues, including fasciae, contributing to complications such as diabetic foot ulcers. Molecular characterization of fascial alterations in T2DM remains limited. Fourier transform infrared (FTIR) spectroscopy enables label-free biochemical profiling of multiple macromolecular classes, potentially addressing limitations of conventional histochemical methods. This study aimed to characterize T2DM-associated biomolecular changes in human deep fasciae using attenuated total reflectance (ATR)-FTIR spectroscopy and chemometrics. Methods ATR-FTIR spectra were obtained from wet postmortem samples of fascia lata, thoracolumbar fascia, and plantar fascia from 18 male subjects with T2DM and 18 non-diabetic controls. Multi-stage multivariate curve resolution-alternating least squares (MCR-ALS) identified spectral components and their proportions. Complementary semiquantitative histochemical analyses of selected macromolecules were also performed. Results MCR-ALS resolved FTIR spectra into five components: collagen-dominant dense/ordered (SC1) and less ordered/loose (SC2) structures, ground substance (SC3), lipids (SC4), and water (SCBW). Significant differences in SC1, SC4, and SCBW contributions were detected across fascia types. T2DM samples showed significantly reduced ground substance (SC3) in thoracolumbar fascia, with trends toward lower dense collagen and increased SC2/SC1 ratio most evident in this region. Histochemistry showed decreased glycosaminoglycans (plantar fascia) and PAS-positive polysaccharides/glycoconjugates (thoracolumbar fascia) in T2DM, with elastin varying by fascia type but not diabetes status. Conclusion ATR-FTIR spectroscopy combined with MCR-ALS characterized region-dependent macromolecular profiles in human deep fascia and identified a T2DM-associated reduction in a ground-substance-associated component in thoracolumbar fascia. This approach offers a promising method to investigate connective tissue biomolecular alterations in metabolic diseases. deep fascia type 2 diabetes mellitus fourier transform infrared spectroscopy FTIR globoka fascija sladkorna bolezen tipa 2 infrardeča spektroskopija s Fourierovo transformacijo true false true Angleški jezik Slovenski jezik Članek v reviji 2026-07-21 07:48:01 2026-07-21 07:48:15 2026-07-31 12:16:27 0000-00-00 00:00:00 2026 0 0 17 str. art. 103642 Vol. 28 Avg. 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.4 2211-7156 10.1016/j.rechem.2026.103642 285437443 RAZ_Ugwoke_Chiedozie_Kenneth_2026.pdf RAZ_Ugwoke_Chiedozie_Kenneth_2026.pdf 1 E2F9820103957CCE02E516092934E7D0 87f4d6ff3793472aa024f0e48af2db87528eb5d4f72125e3f4dd837b941874c2 8d5a5d51-84c8-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=240523 https://www.sciencedirect.com/science/article/pii/S2211715626006168?via%3Dihub 1 87d4a697-84c7-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=240522 Medicinska fakulteta Biotehniška fakulteta 0 0 0