20.500.12556/RUL-185006 Preventing moisture condensation on Petri dish lids Moisture condensation on the lids of Petri dishes (PDs) during incubation is a common issue in microbiological laboratories, which affects microbiological water quality testing. This research focuses on the condensation problem during the use of Reasoner’s agar (R2A) in water testing for pharmaceutical applications. Condensation can distort microbial colony counts that potentially compromise the reliability of test results. The findings presented in this work are limited to the investigated incubation conditions and R2A agar systems. We conducted a detailed investigation into the factors contributing to condensation, particularly temperature gradients and relative humidity within vertically stacked PDs during incubation. Our experiments revealed that condensation is primarily driven by temperature differences between adjacent PDs, with higher condensation levels forming in dishes at the top of the stack. We explored various strategies, including improved air circulation and thermal insulation, but found that horizontal stacking of PDs effectively prevented condensation. These findings offer a viable method to enhance the accuracy of microbiological testing in pharmaceutical environments by mitigating moisture-related issues. Petri dishes condensation microbiological water testing reasoner’s agar incubation conditions temperature gradients Petrijevke kondenzacija mikrobiološko testiranje vode Reasoner agar inkubacijski pogoji temperaturni gradienti true false true Angleški jezik Slovenski jezik Članek v reviji 2026-07-20 13:06:08 2026-07-20 13:06:12 2026-07-21 04:02:46 0000-00-00 00:00:00 2026 0 0 9 str. no. 23, art. 235802 Vol. 37 Jun. 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 621.317:579.67:543.3 0957-0233 10.1088/1361-6501/ae74ff 285431811 Kramer_2026_Meas._Sci._Technol._37_235802.pdf Kramer_2026_Meas._Sci._Technol._37_235802.pdf 1 553F272B4D27FF87A2FB0EDCFAF503FC 74956de4abc0c1bcb34cd01120321c20f289dde0ad535357a3ab04ab8a97acb2 42c1a014-842b-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=240492 https://iopscience.iop.org/article/10.1088/1361-6501/ae74ff 1 cab162e1-842a-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=240491 Fakulteta za elektrotehniko 0 0 0