20.500.12556/RUL-184599 Electrolyzed saline as an alternative to chlorhexidine antimicrobial and biofilm volume outcomes in a 4-day non-brushing randomized controlled clinical trial Background: The biofilm-inhibitory effects of electrolyzed saline (EOS) vary widely due to differences in formulations and treatment methods. This study analyzed the impact of EOS on key oral microbial species and used serial 3D-intraoral scans to evaluate its effects on de novo biofilm formation. Methods: This was a double-blind, randomized, placebo-controlled, cross-over, 4-day, non-brushing, plaque-regrowth study on periodontally healthy individuals. Each subject participated in three trial arms. During each 4-day arm, the subjects refrained from mechanical oral hygiene. Instead, they rinsed their oral cavities twice-daily with EOS, 0.12% chlorhexidine (CHX), or placebo. De novo plaque accumulation after 4 days was assessed as the primary outcome using the Turesky Modification of the Quigley-Hein Plaque Index (TMQHPI) and the volumetric plaque index (VPI). qPCR analyses of key-microbial species and measurements of active-matrix-metalloproteinase-8 (aMMP-8) in gingival crevicular fluid were performed to complement the clinical data. Results: The biofilm-inhibitory effect of CHX was superior to that of EOS at the level of TMQHPlI and VPI, although both significantly reduced biofilms compared with placebo. The broad-spectrum antimicrobial effect of CHX caused significant reductions in overall bacterial loads, while the action of EOS was more selective. Both CHX and EOS markedly reduced the bacterial loads of Tannerella forsythia; CHX remained more effective against Treponema denticola. In contrast, only EOS demonstrated stronger antimicrobial effects against Fusobacterium nucleatum and Prevotella intermedia while showing no significant impact on periodontal commensals. No significant effects on aMMP-8 were observed. Conclusions: EOS showed substantial, but inferior biofilm-inhibitory, effects compared with CHX. However, EOS had more selective and dysbiosis-controlling effects than CHX. The clinical trial was registered at Clintrials.gov under no. NCT05709015. antiseptics chlorhexidine dental biofilm electrolyzed saline mouthwash oral microbiology antiseptiki klorheksidin zobni biofilm elektrolizirana fiziološka raztopina ustna vodica oralna mikrobiologija true false true Angleški jezik Slovenski jezik Članek v reviji 2026-07-10 11:31:38 2026-07-10 11:31:45 2026-07-15 14:12:37 0000-00-00 00:00:00 2026 0 0 Str. 1231-1242 iss. 6 Vol. 97 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.31 0022-3492 10.1002/jper.70070 268126211 Journal_of_Periodontology_-_2026_-_Povsic_-_Electrolyzed_saline_as_an_alternative_to_chlorhexidine__Antimicrobial_and.pdf Journal_of_Periodontology_-_2026_-_Povsic_-_Electrolyzed_saline_as_an_alternative_to_chlorhexidine__Antimicrobial_and.pdf 1 A790262F829AED80FD66E1F7FFB5A54E 96b4df5fc56092779ecb93c4c4992b4d1c42eaa86240de4b6be9f8f661fb1a7a 75d4b036-7f5d-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=237667 https://aap.onlinelibrary.wiley.com/doi/10.1002/jper.70070 1 09d1493e-7c42-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=237511 Medicinska fakulteta 0 0 0