20.500.12556/RUL-184425 Effects of storage temperature on eggshell-associated total aerobic mesophilic bacteria and Salmonella Enteritidis growth and penetration Vpliv temperature shranjevanja na skupno število aerobnih mezofilnih bakterij na jajčni lupini ter rast in penetracijo bakterije Salmonella Enteritidis Although the refrigeration of eggs within the food supply chain is recommended by multiple studies, the official guidelines on egg storage conditions within the food supply chain remain somehow unclear and highly variable among countries. In this study, various microbiological parameters of free-range chicken eggs from a small-scale farm were examined. The aim was to evaluate the influence of different storage temperatures on eggshell microbial load. In addition, the survivability and growth kinetics of Salmonella enterica serovar Enteritidis (S. Enteritidis) were determined in dependence of the storage temperature. Our results showed significantly higher counts of the total aerobic mesophilic bacteria in eggs stored at 22 °C in comparison to eggs stored at 4 °C. Furthermore, storage at 22 °C supported the growth kinetics of S. Enteritidis on the eggshell as well as its penetration to the inner shell membranes. In contrast, storage at 4 °C prevented the replication of S. Enteritidis on the eggshell and its penetration into the internal egg contents. Consistent with the findings of other studies, our results clearly demonstrate the benefits of eggs refrigeration during transport and storage. table eggs storage storage temperature microbiology aerobic mesophilic bacteria Salmonella Enteritidis food safety jedilna jajca shranjevanje temperatura shranjevanja mikrobiologija aerobne mezofilne bakterije Salmonella enteritidis prehranska varnost true false true Angleški jezik Slovenski jezik Članek v reviji 2026-07-07 09:40:46 2026-07-07 09:40:49 2026-07-08 04:12:18 0000-00-00 00:00:00 2026 0 0 8 str. no. 2 Vol. 122 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 63 1854-1941 10.14720/aas.2026.122.2.24619 283838723 24619_Ozanic+Bucar+et+al_122-2-2026.pdf 24619_Ozanic+Bucar+et+al_122-2-2026.pdf 1 AB6B20A6069E9A576EFA435E13980DC9 e42a207817c551351fc85c58cfdad94fd50db06e3f3dfa897b53d8f2a577abbd 340215bb-79d7-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=237215 https://journals.uni-lj.si/aas/article/view/24619 1 0f510054-79d7-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=237214 Biotehniška fakulteta Acta agriculturae Slovenica 0 0 0