20.500.12556/RUL-184338 Uporaba regulatorjev razvoja za izboljšanje in vitro regeneracije po transformaciji rastlin Use of developmental regulators to improve in vitro regeneration of plant transformation Genska transformacija rastlin je trenutno ena najpomembnejših metod v rastlinski biotehnologiji, ki je omogočila izboljšanje lastnosti številnih kmetijsko pomembnih rastlin. Kljub številnim napredkom te tehnike pa je za določene vrste in njihove genotipe zelo neučinkovita ali pa nemogoča. Uspešnost transformacije je poleg učinkovitega stabilnega vnosa željene DNA v veliki meri odvisna od sposobnosti regeneracije kalusnih tkiv v funkcionalne rastline. In vitro regeneracija rastlin zaradi kompleksnosti regulacije somatske embriogeneze in de novo organogeneze z uporabo hormonov pri nekaterih genotipih ne omogoča učinkovitega razvoja odraslih rastlin. Raziskave teh sistemov so identificirale ključne gene, ki regulirajo te procese in spodbujajo regeneracijo. Tako poimenovani regulatorji razvoja so široka skupina signalnih molekul, ki nadzorujejo diferenciacijo in dediferenciacijo rastlinskih tkiv, njihovo rast ter odziv na abiotske in biotske dejavnike. Trenutno najbolj raziskan je vpliv vnosa genov kot so GRF (ang. Growth regulating factor), BBM (ang. BABY BOOM) in WUS (ang. Wuschel) s katerimi so dokazali spodbujanje regeneracijske sposobnosti transformiranih celic in posledično izboljšano uspešnost transformacije. Genetic transformation of plants is currently one of the most important methods in plant biotechnology, that has improved the development of favorable traits of numerous agriculturally important plants. Despite many advances of this technique, it remains highly inefficient or even impossible for certain species and their genotypes. The success of transformation, besides the effective stable integration of the desired DNA, is largely dependent on the ability of callus tissues to regenerate into functional plants. Due to the complexity of regulating somatic embryogenesis and de novo organogenesis with hormones, in vitro regeneration of plants does not allow for the efficient development of adult plants. Research of these systems has identified key genes that regulate these processes and promote regeneration. These genes were termed as developmental regulators and are a broad group of signaling molecules that control the differentiation and dedifferentiation of plant tissues, their growth, and their response to abiotic and biotic factors. Currently, the most researched effect is the genetic transformation with these genes such as GRF (Growth regulating factor), BBM (BABY BOOM), and WUS (Wuschel), which have been shown to improve the regenerative capacity of callus tissue and consequently the success of transformation. In vitro regeneracija transformacija rastlin A. tumefaciens regulatorji razvoja In vitro regeneration plant transformation A. tumefaciens developmental regulators true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2026-07-05 07:15:57 2026-07-05 07:16:07 2026-07-07 10:29:55 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 280498 283862019 58086.pdf 58086.pdf 1 3334CBF31CE52BDCF9230A7CCABA4AE6 c011d1980ddaa93c318bc8b815d380eb5dc780f8bf3c3e933b1f2774f5ac4cfd 834662a7-7830-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=237018 Biotehniška fakulteta 0 0 0