20.500.12556/RUL-184286 Identification of potential biomarkers for colorectal cancer in a mouse model using proteomics approaches research data underlying the doctoral dissertation Odkrivanje možnih biooznačevalcev raka debelega črevesa in danke pri mišjem modelu s proteomskimi pristopi raziskovalni podatki, obravnavani v doktorskem delu Early inflammation-driven tumorigenesis causes significant remodeling of the colonic extracellular matrix, making its proteomic composition an underexplored source of biomarker candidates. In this study, we characterized the proteomes of healthy and cancerous decellularized mouse colons and compared them to native tissues, confirming the decellularization enhances extracellular matrix protein detection. Colitis-associated colorectal cancer was modeled using the azoxymethane and dextran sulfate sodium (AOM/DSS) mouse model. Colon tissues were decellularized and analyzed using mass spectrometry. Proteomic profiling of decellularized tissues identified 26 proteins upregulated in the AOM/DSS group. Among these, three proteins were not previously proposed as stool biomarkers. Two candidates, neutrophilic granule protein and interferon-induced transmembrane protein 3, were confirmed as upregulated in mice stool samples using ELISA. Their performance characteristics were evaluated against clinically used stool biomarkers, haemoglobin and faecal calprotectin, showing complementary results in multi-protein panels for discriminating between early tumorigenic stages and healthy controls, and in discerning inflammation-only changes. These findings demonstrate that decellularized colon proteomics are highly valuable for identifying previously unrecognized stool-detectable proteins that show potential as non-invasive early colorectal cancer biomarkers. Zgodnja tumorigeneza, ki jo povzroča vnetje, povzroča znatno preoblikovanje zunajceličnega matriksa debelega črevesa, zaradi česar njegova proteomska sestava predstavlja primeren vir za odkrivanje možnih bioloških označevalcev. V tej študiji smo okarakterizirali proteome zdravih in rakavih decelulariziranih mišjih debelih čreves ter jih primerjali z nativnimi tkivi, s čimer smo potrdili, da decelularizacija izboljša zaznavanje proteinov zunajceličnega matriksa. S kolitisom povezan rak debelega črevesa in danke smo povzročili na mišjem modelu z uporabo azoksimetana in natrijevega dekstran sulfata (AOM/DSS). Tkiva debelega črevesa smo decelularizirali in analizirali z masno spektrometrijo. S proteomskim profiliranjem decelulariziranih tkiv smo identificirali 26 beljakovin, ki so bili povišano izraženi v skupini AOM/DSS. Med temi, trije proteini predhodno niso bili predlagani kot biooznačevalci v blatu. Za dva kandidata, nevtrofilni granularni protein in z interferonom induciran transmembranski protein 3, je bilo s testom ELISA potrjeno, da sta v vzorcih blata miši povečano izražena. Njune zmogljivostne značilnosti smo ovrednotili v primerjavi s klinično uporabljenimi biooznačevalci v blatu, hemoglobinom in fekalnim kalprotektinom, pri čemer so večproteinski paneli pokazali komplementarne rezultate pri razlikovanju med zgodnjimi tumorigenimi stadiji in zdravimi kontrolami ter pri zaznavanju sprememb, ki so posledica zgolj vnetja. Te ugotovitve dokazujejo, da je proteomska analiza decelulariziranega debelega črevesa zelo dragocena za prepoznavanje predhodno neznanih proteinov, zaznavnih v blatu, ki kažejo potencial kot neinvazivni zgodnji biooznačevalci raka debelega črevesa in danke. extracellular matrix decellularization proteomics stool biomarker colorectal cancer AOM/DSS mouse model zunajcelični matriks decelularizacija proteomika biooznačevalec v blatu rak debelega črevesa in danke AOM/DSS mišji model true false false Angleški jezik Slovenski jezik 2021-2026 Neznano 2026-07-03 07:52:22 2026-07-14 14:52:17 2026-07-16 10:17:32 0000-00-00 00:00:00 2026 0 0 The 2,078 listed files are organized into the following folders / Navedenih 2.078 datotek je organiziranih v naslednje mape: - AOMDSS experiments, - Decellularization, - ELISA experiments, - SDS PAGE gels for MS, - Western blot experiments. 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 2026-10-01 README.docx README.docx README 1 B35CF2A852F81BD4AC72005871506B2A cae834e1de14b6859bf773436b3208ed88d347bad1d0c0eb08afef31f42c5d69 884ae14d-7f87-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=239778 Medicinska fakulteta Poskus Poskus: Laboratorij Meritve in testi: Fizično 2078 0 0