20.500.12556/RUL-184281 Vpliv aerobne vadbe z omejenim pretokom krvi na kinetiko kisika v skeletni mišici magistrsko delo Effect of blood flow-restricted aerobic exercise on skeletal muscle oxygen kinetics master's thesis Uvod: Mehanizmi prilagoditve skeletnih mišic na vadbo z omejenim pretokom krvi v aktivnih skeletnih mišicah z napihljivo manšeto (ishemična vadba) še niso podrobno raziskani. Predvideva se, da je ključni sprožilec prilagoditve lokalna hipoksija skeletnih mišic. Namen: Namen naše raziskave je bil ovrednotiti učinek ishemične aerobne vadbe na kinetiko kisika in prekrvljenost štiriglave stegenske mišice med kolesarjenjem in v 10-minutnem obdobju okrevanja po njem. Metode dela: Zdravi preiskovanci (n=21), stari med 18–35 let, so opravili tri testiranja na cikloergometru. Prvič smo s tristopenjskim testom določili maksimalno aerobno izhodno moč (Pmaks), nato pa dvakrat izvedli dvostopenjski test (40% in 60% Pmaks), in sicer enkrat z omejitvijo krvnega pretoka v stegenskih mišicah (ishemična vadba) in drugič brez (kontrolna vadba). Med kolesarjenjem in med okrevanjem po njem smo v mišicah v. lateralis in v. medialis s spektroskopijo blizu infrardečega spektra neprekinjeno merili spremembe v koncentraciji skupnega hemoglobina ([tHb]), razlike v koncentraciji oksigeniranega in deoksigeniranega hemoglobina ([Hbdiff]) in razlike v indeksu saturacije tkiva (TSI). Rezultati: Analiza interakcije časa in intervencije z ANOVA je pokazala, da se je [tHb] v obeh mišicah med ishemično vadbo pomembno bolj (p < 0,05) povečala kot med kontrolno vadbo. Tudi med okrevanjem po ishemični vadbi je bil opazen trend povišane [tHb], vendar je bila razlika s kontrolno vadbo značilna le prvih nekaj minut. TSI in [Hbdiff] sta se v mišici v. lateralis med ishemično vadbo pomembno bolj znižala (p < 0,05) kot med kontrolno vadbo, med tem ko v mišici v. medialis interakcija ni bila značilna. Nižje vrednosti [Hbdiff] v obeh mišicah so bile prisotne tudi prvih 2–6 minut okrevanja po ishemični vadbi (p < 0,05) v primerjavi s kontrolno vadbo, nato so razlike izzvenele. Nasprotno, interakcija sprememb TSI v mišici v. medialis med in po vadbi ni bila značilna. Razprava in zaključek: Rezultati kažejo, da mehanski pritisk napihljive manšete zmanjša prekrvljenost in povzroči tkivno hipoksijo mišice v. lateralis med kolesarjenjem, kar verjetno povzroči zgodnejše vklaplanje anaerobne presnove in posledično večji kisikov dolg v obdobju okrevanja po vadbi. Podoben, a manj značilen učinek, je bil nakazan tudi za mišico v. medialis. Potrebne so nadaljne raziskave na večjem vzorcu. Introduction: The mechanisms by which skeletal muscles adapt to restricted blood flow during activity using an inflatable cuff (ischemic exercise) have not yet been thoroughly investigated. However, it is assumed that the key stimulus is localized skeletal muscle hypoxia. Purpose: The aim of this study was to evaluate the effects of ischemic aerobic exercise on oxygen kinetics and blood perfusion in the quadriceps muscle during cycling and during a 10-minute early post-exercise recovery phase. Methods: Healthy participants (n = 21), aged 18–35 years, completed three trials on a stationary bike. First, maximal oxygen consumption (Pmax) was determined using a three-stage test. Participants then performed two separate two-stage tests (at 40% and 60% of Pmax), once with blood flow restriction applied to the quadriceps muscle (ischemic exercise) and once without blood flow restriction (control exercise). During cycling and recovery, changes in total hemoglobin concentration ([tHb]), the difference between oxygenated and deoxygenated hemoglobin ([Hbdiff]), and the tissue saturation index (TSI) were continuously measured in the vastus lateralis and vastus medialis muscles using near-infrared spectroscopy. Results: Analysis of the interaction between time and intervention using ANOVA showed that [tHb] in both muscles increased significantly more (p < 0.05) during ischemic exercise compared to control exercise. During recovery following ischemic exercise, a trend toward elevated [tHb] was also observed; however, the difference compared with control exercise was significant only in the first few minutes. TSI and [Hbdiff] in the vastus lateralis muscle decreased significantly more (p < 0.05) during ischemic exercise than during control exercise, whereas no significant interaction was found in the vastus medialis muscle. Lower [Hbdiff] values in both muscles were also observed during the first 2–6 minutes of recovery following ischemic exercise (p < 0.05) compared to control exercise, after which the differences disappeared. In contrast, no significant interaction was observed for TSI changes in the vastus medialis muscle during or after exercise. Discussion and Conclusion: The findings indicate that the mechanical pressure of the inflatable cuff reduces blood flow and induces tissue hypoxia in the vastus lateralis muscle during cycling. This likely leads to the earlier activation of anaerobic metabolism and, consequently, a greater oxygen deficit during recovery. A similar but less pronounced effect was observed in the vastus medialis muscle. Further studies with a larger sample size are needed. magistrska dela fizioterapija kinetika kisika vadba z omejenim pretokom krvi ishemična vadba aerobna vadba mišična oksigenacija NIRS master's theses physiotherapy oxygen kinetics blood flow restriction training ischemic exercise aerobic exercise muscle oxygenation NIRS true false false [K. Čepon] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2026-07-03 07:45:36 2026-07-03 07:45:45 2026-07-04 04:21:55 0000-00-00 00:00:00 2026 0 Ljubljana 0 Ljubljana 54 str., [10] str. pril. 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615.8 160237 283498499 59301.pdf 59301.pdf 1 362FB22C8535B11E6206071CDFD9B417 9750b9b73404a8c1e79e2d75d3980e25317ab10fbfb94df0969e4b6f60007f32 55a5677a-76a2-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=236920 Zdravstvena fakulteta 0 0 0