20.500.12556/RUL-184161 Vpliv spektralnih interferenc na absorpcijski spekter makrokomponente v kislem mediju Influence of Spectral Interferences on Absorption Spectrum of Macrocomponent in Acidic Medium V diplomskem delu sem z molekularno absorpcijsko spektrometrijo v ultravijoličnem področju raziskovala vpliv spektralnih interferenc na absorpcijski spekter makrokomponent v kislem mediju. Kot modelni sistem sem uporabila trinatrijev citrat. Posebno pozornost sem namenila spremembam oblike spektra v območju med 200 in 230 nm, kjer literatura poroča o možnem vplivu dodatnih komponent v citratnih epruvetah. Analizirala sem absorpcijske spektre citrata ter različnih potencialnih interferenc, med katerimi so bili nitrat, nitrit, oksalat, format, acetat, bromid in klorid. Spektralne lastnosti posameznim komponent sem primerjala s spektrom trinatrijevega citrata ter ovrednotila njihov možen vpliv na obliko absorpcijskega pasu. Meritve sem izvajala v HCl mediju 126 mmol/L, pri čemer sem proučila tudi vpliv medija na obliko spektra. Za boljšo ločitev med komponentami sem poleg spektrov ničtega reda uporabila tudi prvi odvod absorpcijskih spektrov. Primerjava odvodov je omogočila natančnejše vrednotenje sprememb v obliki spektra, predvsem razlik v položaju ekstremov, ničelnih prehodov in naklonov krivulj. Rezultati so pokazali, da nekatere interferenčne komponente povzročijo predvsem povečanje absorbance pri maksimumu absorpcije citrata. V zaključnem delu sem analizirala tudi spektre citratnih raztopin iz različnih epruvet ter jih primerjala s spektrom referenčnega citrata. Diferencialna spektroskopija se je izkazala kot uporabna metoda za zaznavo spektralnih interferenc in vrednotenje sprememb oblike spektrov v kompleksnih sistemih. In this bachelor's thesis, I investigated the influence of spectral interferences on the absorption spectrum of macrocomponents in an acidic medium using molecular absorption spectrometry in ultraviolet range. Trisodium citrate was used as the model system. Particular attention was devoted to changes in spectral shape in the wavelength range between 200 and 230 nm, where literature reports the possible influence of additional components present in citrate blood collection tubes. The absorption spectra of citrate and various potential interfering components were analyzed, including nitrate, nitrite, oxalate, formate, acetate, bromide and chloride. The spectral properties of individual components were compared with the spectrum of trisodium citrate, and their potential influence on the shape of the absorption band was evaluated. Measurements were performed in a 126 mmol/L HCl medium, and the influence of the medium itself on spectral shape was also investigated. To improve the differentiation between individual components, first-derivative absorption spectra were analyzed in addition to the zero-order spectra. Comparison of derivative spectra enabled a more precise evaluation of spectral shape changes, particularly differences in the positions of extrema, zero-crossings, and curve slopes. The results showed that some interfering components primarily caused an increase in absorbance at the citrate absorption maximum. In the final part of the study, absorption spectra of citrate solutions prepared from different blood collection tubes were also analyzed and compared with the spectrum of reference citrate. Differential spectroscopy proved to be a useful approach for detecting spectral interferences and evaluating changes in the shape of spectra in complex systems. molekularna absorpcijska spektrometrija ultravijolično področje trinatrijev citrat spektralne interference diferencialna spektroskopija molecular absorption spectrometry ultraviolet range trisodium citrate spectral interferences differential spectroscopy true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2026-07-01 09:50:04 2026-07-01 09:50:09 2026-07-30 13:44:15 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 25938 286478595 Cvelfer_Tita_-_Vpliv_spektralnih_interferenc_na_absorpcijski_spekter_makrokomponente_v_kislem_me.pdf Cvelfer_Tita_-_Vpliv_spektralnih_interferenc_na_absorpcijski_spekter_makrokomponente_v_kislem_me.pdf 1 C1A41E8C0CF42E982DC89759EF636BDE 1ac7db8798bd1de023acf5ad4ab6dd1020c9a5c70494e101c460921e6514e61b 601dcb54-7521-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=236693 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0