20.500.12556/RUL-183942 A novel hexasegmented virus isolated from the phytopathogenic fungus Verticillium nonalfalfae In this study, we describe a novel virus, tentatively named Verticillium nonalfalfae virus M (VnaVM), identified in the phytopathogenic fungus Verticillium nonalfalfae isolated from hop (Humulus lupulus L.). The VnaVM genome consists of six double-stranded RNA (dsRNA) segments ranging from 1051 to 2401 bp. DsRNAs 1–3 encode an RNA-dependent RNA polymerase (RdRp), a hypothetical protein and a methyltransferase (MTR), respectively, while dsRNA6 encodes a proline-alanine-serine-rich protein (PASrp). These four proteins share less than 58% amino acid identity with homologs encoded by viruses in the family Polymycoviridae. Products encoded by dsRNAs 4 and 5 show no similarity to known proteins, and their roles in the viral life cycle are unknown. Phylogenetic analysis of RdRp amino acid sequences, together with genome organization and pairwise identity values, supports classifying VnaVM as a representative of a novel species within the genus Multimycovirus (family Polymycoviridae). To our knowledge, this is the first report of a polymycovirid infection in a member of the genus Verticillium. Verticillium nonalfalfae phylogenetic analysis virus identification phytopathogenic fungus Humulus lupulus virusi Verticillium nonalfalfae virus M fitopatogene glive filogenetska analiza true false true Angleški jezik Slovenski jezik Članek v reviji 2026-06-22 14:40:57 2026-06-22 14:41:03 2026-06-23 04:01:00 0000-00-00 00:00:00 2026 0 0 6 str. iss. 7, art. 216 Vol. 171 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 0304-8608 282475011 s00705-026-06682-6.pdf s00705-026-06682-6.pdf 1 550C3BD7A7B541118C4B61EA910127F9 00fbd4a7754354bb741460110244330e119606d82f8c63d628da82b9a2f08214 0f4da6b5-6e38-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=236276 https://link.springer.com/article/10.1007/s00705-026-06682-6 0 229d5e06-6e38-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=236277 Biotehniška fakulteta 0 0 0