20.500.12556/RUL-183771 nosZII communities influence soil N2O consumption potential in a long-term tillage and fertilization experiment Emissions of N2O have an important environmental impact, with fertilization in agricultural systems contributing a substantial part. Understanding how different agricultural practices influence microbial processes governing N2O production and consumption is therefore urgently needed. This study aims to elucidate the underlying mechanisms driving N2O emissions in experimental plots subject to a combination of different tillage and fertilization practices. N2O consumption potential was evaluated in soil sampled from the field experiment by determining the genetic potential for N2O consumption/production and by measuring potential N2O production and denitrification activity (PDA) assays with and without carbon (C) substrate. The highest N2O consumption potential was observed in soil from organically fertilized no-till treatment. Addition of C to the PDA assay significantly increased N2O consumption in conventionally tilled soil, which exhibited higher proportion of nosZII genes, coding for clade II nitrous oxide reductase, in the metagenome, and a more diverse microbial community. Correspondingly, the proportion and abundance of nosZII genes as well as microbial diversity were significantly and positively related to N2O consumption potential. Gradients in soil physical and chemical properties reflected the tillage-driven differences in the composition of microbial communities, which however did not correlate with N2O consumption potential. Our findings suggest that overall taxonomy-based community structure had less importance for N2O consumption potential. Instead, the negative correlation between nosZII gene and the N2O/(N2O + N2) ratio emphasises the need to examine microbial functional capacity for better prediction of process outcomes. denitrification denitrifiers nirK nirS nosZI nosZII mikrobni procesi denitrifikacija N2O biologija tal obdelava tal gnojenje prakse upravljanja tal true false true Angleški jezik Slovenski jezik Članek v reviji 2026-06-18 12:33:58 2026-06-18 12:34:10 2026-06-19 04:14:14 0000-00-00 00:00:00 2026 0 0 10 str. art. 107165 Vol. 224 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 631.4 0929-1393 282091011 1-s2.0-S0929139326003859-main.pdf 1-s2.0-S0929139326003859-main.pdf 1 D4E7E3234C67D99D1BE4CCD42CF9CDFF ff36e30c6490470933cd2e89ede2ad615dd8ea0c52eb5745d1199d92afbdf4ab 9345bb47-6b02-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=235946 https://www.sciencedirect.com/science/article/pii/S0929139326003859 1 2e2d270b-6b01-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=235945 Biotehniška fakulteta 0 0 0