20.500.12556/RUL-183541 Herpesviridae and atypical bacteria co-detections in lower respiratory tract samples of SARS-CoV-2-positive patients admitted to an intensive care unit Shortly after the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), cases of viral, bacterial, and fungal coinfections in hospitalized patients became evident. This retrospective study investigates the prevalence of multiple pathogen co-detections in 1472 lower respiratory tract (LRT) samples from 229 SARS-CoV-2-positive patients treated in the largest intensive care unit (ICU) in Slovenia. In addition to SARS-CoV-2, (rt)RT-PCR tests were used to detect cytomegalovirus (CMV), Epstein–Barr virus (EBV), herpes simplex virus 1 (HSV-1), herpes simplex virus 2 (HSV-2), varicella zoster virus (VZV), and atypical bacteria: Chlamydia pneumoniae, Mycoplasma pneumoniae and Legionella pneumophila/spp. At least one co-detection was observed in 89.1% of patients. EBV, HSV-1, and CMV were the most common, with 74.7%, 58.1%, and 38.0% of positive patients, respectively. The median detection time of EBV, HSV-1, and CMV after initial SARS-CoV-2 confirmation was 11 to 20 days. Bronchoalveolar lavage (BAL) and tracheal aspirate (TA) samples showed equivalent performance for the detection of EBV, CMV, and HSV-1 in patients with both available samples. Our results indicate that SARS-CoV-2 infection could be a risk factor for latent herpesvirus reactivation, especially HSV-1, EBV, and CMV. However, additional studies are needed to elucidate the clinical importance of these findings. SARS-CoV-2 co-detections coinfections lower respiratory tract ICU herpesviruses atypical bacteria SARS-CoV-2 soodkrivanje sočasne okužbe spodnji dihalni trakt herpesvirusi atipične bakterije true false true Angleški jezik Slovenski jezik Članek v reviji 2026-06-14 06:35:44 2026-06-18 09:27:19 2026-07-23 07:37:37 0000-00-00 00:00:00 2024 0 0 14 str. iss. 4, art. 714 Vol. 12 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 578 2076-2607 10.3390/microorganisms12040714 192370691 RAZ_Grubelnik_Gasper_2024.pdf RAZ_Grubelnik_Gasper_2024.pdf 1 9096CBA69D1DE657985DC08185C770B3 71f1879aded505e6b19c07089c4caeba4b48c7fc80eee380e02b54b1002fc892 8e562345-6ae7-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=235925 https://www.mdpi.com/2076-2607/12/4/714 1 7938b5e6-67aa-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=235604 Medicinska fakulteta 0 0 0