20.500.12556/RUL-183256 Targeting resistant Staphylococcus aureus biofilm with organic acids uncovering the biofilm disinfection mechanism This study examines the effects of ascorbic, acetic, citric, and lactic acids on resistant S. aureus, assessing planktonic growth using minimal inhibitory (MIC) and bactericidal concentrations, and evaluating mature biofilms for viability, biomass, enzyme activity, membrane integrity and stress. We found the lowest antimicrobial potential for acetic acid, followed by ascorbic acid, citric acid and lactic acid. Treatment of mature biofilms showed a reduction of up to 3 log CFU mL$^{−1}$ for lactic acid, while other organic acids were less effective. Lactic acid was also the most effective in reducing biofilm biomass by up to 33%, indicating potential for combination with other antibacterial compounds. The crystal violet staining confirmed a reduction in biomass regarding the non-treated samples. Iodonitrotetrazolium chloride assay showed a decrease in metabolic activity, with the highest formazan reduction (88%) observed with acetic acid. Live/dead staining indicated increased cell death at higher concentrations (9 MIC), with lactic acid causing the most severe membrane damage. In addition, intracellular stress increased with acid concentration. These findings reveal not only differential biofilm-targeting effects among common organic acids but also highlight the translational potential of lactic and acetic acids as cost-effective strategies to control resistant S. aureus in clinical and industrial settings, providing a foundation for future therapeutic and preventive applications. resistant S. aureus organic acids biofilm acetic and lactic acids true false true Angleški jezik Ni določen Članek v reviji 2026-06-09 14:22:50 2026-06-09 14:22:51 2026-09-29 04:06:19 0000-00-00 00:00:00 2026 0 0 Str. 446-458 no. 4 Vol. 42 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 544.023.22:615.015.1 0892-7014 10.1080/08927014.2026.2634396 269559555 Targeting_resistant_Staphylococcus_aureus_biofilm_with_organic_acids__uncovering_the_biofilm_disinfection_mechanism.pdf Targeting_resistant_Staphylococcus_aureus_biofilm_with_organic_acids__uncovering_the_biofilm_disinfection_mechanism.pdf 1 3FFCD2371BB922CD8155FB8FC4B72D47 f95c71c614f0e34d47c5be902b1533f938311e154f540d02af31fd6d0532a704 adf4289e-bb36-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=249144 https://www.tandfonline.com/doi/full/10.1080/08927014.2026.2634396 0 57824518-63fe-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=235213 Zdravstvena fakulteta 0 0 0