20.500.12556/RUL-183079 Human endothelium-on-a-chip development of a microfluidic model for cell viability assessment under oxidative injury Endothelial responses to oxidative stress are influenced by the presence of blood flow and shear stress. To better capture these conditions in vitro, we developed an endothelium-on-a-chip model for the study of acute oxidative injury in EA.hy926 cells under flow. We adapted the culture conditions for use outside a conventional 5% CO$_2$ atmosphere, and we improved endothelial adhesion and retention under flow by collagen coating of the chip surface. Our model tolerated shear stress of up to 0.89 Pa, which corresponds to the in vivo values experienced by endothelial cells in the aorta and venous system. On this model, we designed an acute oxidative injury protocol based on high concentrations of hydrogen peroxide ($H_{2O_2}$) as an endogenous source of oxidative stress and 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH) as an exogenous molecule. The inclusion of flow increased the apparent sensitivity of the model to oxidative injury compared to static conditions, as the IC$_{50}$ values decreased from 16.2 mM to 12.0 mM for ($H_{2O_2}$) and from 100.7 mM to 72.5 mM for AAPH under flow conditions. Pretreatment with quercetin (1.5 µM) reduced AAPH-induced oxidative injury, thus showing the potential applicability of the model for antioxidant screening assays. endothelium organ-on-a-chip microfluidics oxidative stress shear stress cell viability organ na čipu mikrofluidika strižna napetost celična viabilnost oksidativni stres endotelij true false true Angleški jezik Slovenski jezik Članek v reviji 2026-06-04 06:54:21 2026-06-04 06:54:30 2026-06-07 03:16:27 0000-00-00 00:00:00 2026 0 0 20 str. iss. 11, art. 5018 Vol. 27 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 612.015+611.018.74 1422-0067 10.3390/ijms27115018 280213251 ijms-27-05018.pdf ijms-27-05018.pdf 1 C1C57BB7011E74CE45EEB740EC468F4B 8fa890cf1c89747f195227cc1cbab61d8a8541b1411aad314fba6a4b44c9d8b4 a21e72d4-5fd1-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=234920 https://www.mdpi.com/1422-0067/27/11/5018 1 22be681e-5fd1-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=234919 Fakulteta za farmacijo Medicinska fakulteta 0 0 0