20.500.12556/RUL-182459 Molecular screening for cyanobacteria and their cyanotoxin potential in diverse habitats research data underlying the article Pregled cianobakterij in njihovega potenciala za proizvodnjo cianotoksinov v raznolikih habitatih raziskovalni podatki, obravnavani v članku Cyanobacteria are adaptable and dominant organisms that exist in many harsh and extreme environments due to their great ecological tolerance. They produce various secondary metabolites, including cyanotoxins. While cyanobacteria are well studied in surface waters and some aerial habitats, numerous other habitats and niches remain underexplored. We collected 61 samples of: (i) biofilms from springs, (ii) aerial microbial mats from buildings and subaerial mats from caves, and (iii) water from borehole wells, caves, alkaline, saline, sulphidic, thermal, and iron springs, rivers, seas, and melted cave ice from five countries (Croatia, Georgia, Italy, Serbia, and Slovenia). We used (q)PCR to detect cyanobacteria (phycocyanin intergenic spacer—PC-IGS and cyanobacteria-specific 16S rRNA gene) and cyanotoxin genes (microcystins—mcyE, saxitoxins—sxtA, cylindrospermopsins—cyrJ), as well as amplicon sequencing and morphological observations for taxonomic identification. Cyanobacteria were detected in samples from caves, a saline spring, and an alkaline spring. While mcyE or sxtA genes were not observed in any sample, cyrJ results showed the presence of a potential cylindrospermopsin producer in a biofilm from a sulphidic spring in Slovenia. This study contributes to our understanding of cyanobacteria occurrence in diverse habitats, including rare and extreme ones, and provides relevant methodological considerations for future research in such environments. Cianobakterije so prilagodljivi organizmi, ki jih zaradi njihove velike ekološke tolerance najdemo v številnih negostoljubnih in ekstremnih okoljih. Proizvajajo različne sekundarne metabolite, med drugim tudi cianotoksine. Medtem ko so cianobakterije v celinskih vodah in nekaterih kopenskih življenjskih okoljih dobro poznane, so v številnih drugih habitatih in ekoloških nišah še vedno slabo raziskane. V tej raziskavi smo zbrali 61 vzorcev: (i) biofilmov iz izvirov, (ii) mikrobnih prevlek s površin zgradb in iz jam ter (iii) vode iz vrtin, jam, alkalnih, slanih, žveplenih, termalnih in železovih izvirov, rek, morja in jamskega ledu iz petih držav (Hrvaška, Gruzija, Italija, Srbija in Slovenija). Uporabili smo (q)PCR za zaznavanje cianobakterij (fikocianinska medgenska regija PC-IGS in za cianobakterije specifičen gen 16S rRNA) in genov za proizvodnjo cianotoksinov (mikrocistinov – mcyE, saksitoksinov – sxtA, cilindrospermopsinov – cyrJ) ter sekvenciranje amplikonov in morfološko analizo za taksonomsko določevanje cianobakterij. Cianobakterije smo zaznali v vzorcih iz jam in dveh izvirov, slanega ter alkalnega. Genov mcyE ali sxtA nismo opazili v nobenem vzorcu, zaznali pa smo prisotnost potencialnega proizvajalca cilindrospermopsina v biofilmu iz žveplenega izvira v Sloveniji. Ta raziskava prispeva k našemu razumevanju pojavljanja cianobakterij v raznolikih habitatih, vključno z redkimi in ekstremnimi okolji, in izpostavlja nekatere pomembne metodološke izzive za prihodnje raziskave v takih okoljih. extreme environments cylindrospermopsin sulphidic springs caves qPCR PC-IGS PCR DNA sequencing ekstremna okolja cilindrospermopsin žvepleni izvir jame qPCR PC-IGS PCR sekvenciranje DNA true false false Angleški jezik Slovenski jezik Ljubljana, Slovenia From/Od 2020-06-01 to/do 2022-06-17 Neznano 2026-05-12 13:53:54 2026-05-18 09:54:43 2026-05-19 04:36:15 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 README.txt README.txt README 1 580DC1A2F3EA1937DAE2F2443CF7B409 653844d24d332da2332dc589cc66c1a49bdb134eda8b0c86d9bb26e5cc75a5fe 3334e5e0-4df9-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=233746 PREBERIME.txt PREBERIME.txt PREBERI ME 1 6979035EDC193875CD3FB9E04B3CCA23 0e35c729d9d0a6d0f3f18b5a21a34b725af383e8b8bbad237986554bcdf9a48e 32fdace9-4df9-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=233745 data.zip data.zip Data 1 41E9DE2557AA79E3C36990109A225F02 0d06e78140d58b5d8d6bb9275373624750bad7735f7faa7ab7756baaca3190ce 334c638d-4df9-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=233747 https://doi.org/10.3390/toxins16080333 Article 0 270a5424-4df9-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=233744 Biotehniška fakulteta Poskus: Laboratorij Zbiranje Sinteza Molecular screening for cyanobacteria and their cyanotoxin potential in diverse habitats Molecular screening for cyanobacteria and their cyanotoxin potential in diverse habitats 0 0 0