20.500.12556/RUL-182334 Intensification of continuous enzyme-catalyzed furfurylamine synthesis from bio-based furfural using microreactor and deep eutectic solvents research data underlying the article Intenzifikacija kontinuirane encimsko katalizirane sinteze furfurilamina iz biološko pridobljenega furfurala z uporabo mikroreaktorja in globoko evtektičnih topil. raziskovalni podatki, obravnavani v članku Furfurylamine (FA) is an important biomass-derived intermediate with broad applications across many industries. Although biocatalytic routes provide a sustainable alternative to conventional synthesis, their industrial implementation is often limited by low substrate loadings and excessive amine donor requirements. In this study, an intensified continuous-flow biocatalytic process for FA synthesis from furfural (FUR) was developed through the integrating of enzyme N-His6-ATA-wt immobilization on magnetite nanoparticles, reaction medium engineering using deep eutectic solvents (DESs), and microreactor technology. A series of aqueous DESs solutions was systematically screened to improve substrate solubility while preserving enzymatic activity, leading to 49.17 wt% betaine–propylene glycol (1:3) DES in potassium phosphate buffer (pH 6.5, 100 mM), Bet:PG49.17, as the optimal reaction medium. The solubility of the less soluble substrate, (S)-(−)-α-methylbenzylamine (MBA), increased 8.33-fold in Bet:PG49.17 compared to the buffer, while enzymatic activity was fully retained relative to the buffer system (98.98%). Enzyme shelf-life studies demonstrated that the immobilized enzyme exhibited significantly enhanced long-term stability in the DES-containing medium at 25 °C, with a 4.55-fold increase in half-life compared to the immobilized enzyme in buffer. FUR bioamination conducted with an equimolar ratio of the amine donor at a concentration of 100 mM, using enzyme immobilized in a magnetic field-assisted microreactor, achieved a gross yield 87.50% and a space–time yield of 6.43 g/(L h). Moreover, permeation of volatile components through the reactor material enabled in situ removal of inhibitory coproducts, acetophenone, preventing its accumulation and affecting the preserving enzyme activity and in situ removal of the unreacted substrates. furfurylamine biomass valorization deep eutectic solvents green chemistry microreactor flow chemistry true false false Angleški jezik Slovenski jezik Neznano 2026-05-07 12:41:02 2026-05-20 22:57:54 2026-05-21 04:57:07 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 Data.zip Data.zip Data 1 724D57CA154F6D145A01B3239FA73A34 9729e3bfc3264b019535f9375a3bc3902943430ccb521d9f5610ae34f08d5b11 39eb95e5-4a01-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=233496 Fakulteta za kemijo in kemijsko tehnologijo Meritve in testi 0 0 0