20.500.12556/RUL-182160 Vpliv eteričnega olja navadne dobre misli (Origanum vulgare) na kalitev spor in rast glive Penicillium expansum Effect of oregano (Origanum vulgare) essential oil on spore germination and growth of the fungus Penicillium expansum V raziskavi smo proučevali vpliv eteričnih olj (EO) iz dveh populacij navadne dobre misli (Origanum vulgare) na kalitev spor in rast glive Penicillium expansum, pomembnega patogena sadja. Kemijska sestava olj se je bistveno razlikovala po vsebnostih timola in karvakrola, ključnih fungicidnih/fungistatičnih spojinah pri populaciji navadne dobre misli 9/1, ki je vsebovala več teh spojin kot populacija 9/8. Z metodami redčenja in merjenjem polmera rasti micelija smo določili minimalne inhibitorne koncentracije (MIC) in minimalne fungicidne koncentracije (MFC) za populacije EO na dva seva glive P. expansum, »Anka« in »Celar«. EO populacije 9/1 je pokazalo močnejše fungistatične in fungicidne učinke, z vrednostmi MIC med 0,1 in 0,25 μl/ml in MFC do 0,25 μl/ml, medtem ko je bilo za populacijo 9/8 potrebno višje odmerjanje, ali pa ni doseglo MFC. Kalitev spor, polmer rasti micelija in morfologija hif so se bistveno spremenili po izpostavitvi EO, predvsem pri populaciji 9/1, kjer smo opazili tanjšanje hif, povečano številčnost sept in vakuolizacijo, kar kaže na stresni odziv. Te razlike poudarjajo vpliv kemijske variabilnosti EO na protiglivno učinkovitost. Rezultati kažejo, da je EO O. vulgare, zlasti populacije 9/1, obetaven naraven biofungicid za nadzor okužb glive P. expansum. Priporočamo nadaljnje raziskave za optimizacijo formulacij, potrditve učinkovitosti shranjevalnih razmerah ter oceno senzoričnih in fitotoksičnih učinkov za praktično uporabo. In this study, we investigated the antifungal effects of essential oils (EOs) from two populations of Origanum vulgare on spore germination and growth of Penicillium expansum, an important fruit pathogen. The chemical composition of the oils differed significantly in the content of thymol and carvacrol, the key antifungal compounds, in population 9/1, which contained more of these compounds than population 9/8. Using microdilution methods and radial growth measurements, we determined the minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs) of the EO populations on two P. expansum strains, »Anka« in »Celar«. The essential oil of population 9/1 showed stronger fungistatic and fungicidal effects, with MIC values between 0.1 and 0.25 μl/ml and MFC up to 0.25 μl/mL, while population 9/8 required higher dosages and sometimes failed to reach the MFC. Spore germination, growth radius and mycelial morphology were significantly altered after EO treatment, especially in population 9/1, where hyphal thinning, increased septa abundance and vacuolization were observed, indicating a stress response. These differences highlight the influence of chemical variability of EO on antifungal efficacy. The results indicate that EO of O. vulgare, especially population 9/1, is a promising natural biofungicide for the control of infections of P. expansum. Further research is recommended to optimize formulations, confirm the effectiveness of storage conditions and evaluate sensory and phytotoxic effects for practical use. Navadna dobra misel eterično olje Penicillium expansum MIC MFC karvakrol timol Origanum vulgare essential oil Penicillium expansum MIC MFC carvacrol thymol true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2026-04-26 07:15:26 2026-04-26 07:15:35 2026-05-05 13:05:02 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 279419 277077763 57249.pdf 57249.pdf 1 9609F95BFCEE9FECEC91F4B6634E9F8C de0dc62a13534af6de0c78e2e0c0c96ab088238b018ed68e791a617159531634 b1485bae-412e-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=233134 Biotehniška fakulteta 0 0 0