20.500.12556/RUL-182106 Efficient SARS-CoV-2 variant detection and monitoring with Spike Screen next-generation sequencing The emergence and rapid spread of SARS-CoV-2 prompted the global community to identify innovative approaches to diagnose infection and sequence the viral genome because at several points in the pandemic positive case numbers exceeded the laboratory capacity to characterize sufficient samples to adequately respond to the spread of emerging variants. From week 10, 2020, to week 13, 2023, Slovenian routine complete genome sequencing (CGS) surveillance network yielded 41 537 complete genomes and revealed a typical molecular epidemiology with early lineages gradually being replaced by Alpha, Delta, and finally Omicron. We developed a targeted next-generation sequencing based variant surveillance strategy dubbed Spike Screen through sample pooling and selective SARS-CoV-2 spike gene amplification in conjunction with CGS of individual cases to increase throughput and cost-effectiveness. Spike Screen identifies variant of concern (VOC) and variant of interest (VOI) signature mutations, analyses their frequencies in sample pools, and calculates the number of VOCs/VOIs at the population level. The strategy was successfully applied for detection of specific VOC/VOI mutations prior to their confirmation by CGS. Spike Screen complemented CGS efforts with an additional 22 897 samples sequenced in two time periods: between week 42, 2020, and week 24, 2021, and between week 37, 2021, and week 2, 2022. The results showed that Spike Screen can be applied to monitor VOC/VOI mutations among large volumes of samples in settings with limited sequencing capacity through reliable and rapid detection of novel variants at the population level and can serve as a basis for public health policy planning. SARS-CoV-2 COVID-19 next-generation sequencing complete-genome sequencing molecular surveillance spike gene SARS-CoV-2 COVID-19 sekvenciranje naslednje generacije sekvenciranje celotnega genoma molekularni nadzor spike gen true false true Angleški jezik Slovenski jezik Članek v reviji 2026-04-24 10:18:10 2026-04-24 10:18:20 2026-07-20 07:20:03 0000-00-00 00:00:00 2024 0 0 13 str. iss. 4, art. bbae263 Vol. 25 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-097:579.6 1477-4054 10.1093/bib/bbae263 199443203 RAZ_Suljic_Alen_2024.pdf RAZ_Suljic_Alen_2024.pdf 1 F1E0A0A0CF94475A37A882BC187743A7 3d8acc8893e2a27f29cbfbaba4f60408a4da96f6a16a5a80eaf62393dea747ac 853c5b35-3fb6-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=233050 https://academic.oup.com/bib/article/25/4/bbae263/7687614?login=false 1 e787e39a-3fb5-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=233049 Medicinska fakulteta 0 0 0