20.500.12556/RUL-181981 Synthetic biology for designing allostery and its potential biomedical applications Allosteric regulation of protein function, where a perturbation at one site induces a conformational shift or alters dynamics at a distal functional site, plays a key role in numerous biological processes. The ability to introduce allostery using synthetic biology principles holds significant potential both for biomedical and biotechnological applications, and for advancing our understanding of natural allostery. By customizing target proteins for sensing specific chemical or physical signals, including ligand binding and environmental cues, we aim to allosterically modulate the function of a target protein depending on the selected triggers. This approach, unlike active-site targeting, offers greater specificity and selectivity and can allosterically couple diverse physiological processes. Synthetic biology strategies have been developed recently for designed allosteric protein regulation, including the design of allosteric modulators such as domain insertion, generation of de novo allosteric protein switches, and application of engineered allosteric mechanisms to control cellular functions. We examine the application of artificial intelligence (AI)-based generative protein design and other important milestones, challenges and opportunities in this field, highlighting how these approaches could be applied for the development of new therapeutic strategies. allostery protein engineering domain insertion protein switch synthetic biology biokemija beljakovine proteini biološki procesi celične funkcije umetna inteligenca true false true Angleški jezik Slovenski jezik Članek v reviji 2026-04-21 13:48:52 2026-04-21 13:48:53 2026-04-22 04:28:34 0000-00-00 00:00:00 2025 0 0 17 str. iss. 20, art. 169225 Vol. 437 15 October 2025 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 577 1089-8638 10.1016/j.jmb.2025.169225 259537155 1-s2.0-S0022283625002918-main.pdf 1-s2.0-S0022283625002918-main.pdf 1 34BE198B411E219344E016437653BE9D 0ce6cd75e20f4bbb637007b8ea4b751afc67e0c2c261fd76a976fff6b219acf7 4c8e9df2-3d79-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=232830 https://www.sciencedirect.com/science/article/pii/S0022283625002918 1 d46622a2-3d77-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=232829 Medicinska fakulteta 0 0 0