20.500.12556/RUL-181812 Primerjava metod vzorčenja zraka pri določanju mikrobne kontaminacije zraka Comparison of air sampling methods for the determination of microbial air contamination V magistrski nalogi smo analizirali mikrobiološko kakovost zraka v treh laboratorijih z različnimi stopnjami mikrobiološke kontaminacije zraka: Laboratoriju za diagnostiko glivičnih infekcij (GLI), Laboratoriju za hemokulture in druge nujne mikrobiološke preiskave (HEM) in Laboratoriju za sindromsko diagnostiko in molekularno bakteriologijo (SMB). Uporabili smo dve metodi vzorčenja: pasivno metodo IMA (1 urna izpostavitev plošč), EMA (4 urna izpostavitev plošč) in aktivno metodo z zajemom definiranega volumna zraka. Najvišjo mikrobiološko obremenitev smo izmerili v laboratoriju GLI, najnižjo pa v laboratoriju SMB. Med bakterijami so prevladovali rodovi Staphylococcus in Micrococcus, med glivami pa Aspergillus in Penicillium. Ugotovili smo tudi prisotnost laboratorijske kontaminante Chrysonilia sitophila. Statistična analiza s Spearmanovo korelacijo je pokazala statistično značilno povezavo med vzorčenjem IMA in EMA v laboratorijih GLI in HEM, kar kaže na njuno primerljivost v prostorih z višjo mikrobno obremenitvijo. Aktivna metoda je bila bolj občutljiva le v okolju z nižjo mikrobiološko obremenitvijo – v laboratoriju SMB. Zaključimo lahko, da pasivne metode zadostujejo za osnovno oceno mikrobiološke obremenitve prostora z višjim mikrobnim bremenom. Za natančno kvantifikacijo v bolj čistih okoljih pa je priporočljiva uporaba aktivnega vzorčenja. In this master's thesis, we analysed the microbiological air qulity in three laboratories with different levels of microbial contamination: the Laboratory fort he Diagnosis of Fungal Infections (GLI), the Laboratory for Blood Cultures and Other Urgent Microbiological Tests (HEM) and the Laboratory for Syndromic Diagnostics and Molecular Bacteriology (SMB). Two smpling approaches were applied: passive method – IMA (1-hour exposure of agar plates) and EMA (4-hour exposure) – and active method involving the intake of defined volume of air. The highest microbial load was recorded in the GLI laboratory, and the lowest in the SMB laboratory. Among the bacteria, the most prevalent generea were Staphylococcus and Micrococcus, while Aspergillus and Penicillium dominated among the fungi. We also identified the presence of the laboratoy contaminant Chryosonilia sitophila. Statistical analysis using Spearman's correlation showed a statistically significant association between the IMA and EMA methods in the GLI and HEM laboratories, indicating their comparability in environments with higher microbial loads. The active method proved to be more sensitive only in the environment with lower microbial contamination-the SMB laboratory. We conclude that passive methods are sufficient for a basic assessment of microbial air burden in spaces with higher contamination levels. However, for precise quantification in cleaner environments, the use of active sampling is recommended. mikrobiološko vzorčenje zraka aktivna metoda pasivna metoda microbiological air sampling active method passive method true false false [T. Vončina] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2026-04-16 07:15:37 2026-04-16 07:15:46 2026-04-17 04:53:10 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 614.71:614.21:579.083 279348 275455235 57252.pdf 57252.pdf 1 17A78409E28D25069CD41CD381D4CAA2 6c47be62617aa88ffe3a61ccf609a74fbe48fbd0277cc3f72a3af257287c865a 19b14021-3953-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=232539 Biotehniška fakulteta 0 0 0