20.500.12556/RUL-181765 In vitro characterization of probiotic properties of isolated and grown co-cultures on halophyte-based substrate Probiotics are increasingly recognized for their health-promoting effects, yet their performance in unconventional fer mentation systems such as halophyte-based substrates remains poorly understood. Halophytes, salt-tolerant plants rich in phenolics and other bioactive compounds, impose selective pressures that may favor robust and stress-tolerant microor ganisms. In this study, we assessed the probiotic potential of selected lactic acid bacteria and yeast strains, including Lac tococcus (L.) lactis, Bacillus (B.) coagulans ATCC 7050, Saccharomyces (S.) cerevisiae, Kluyveromyces (K.) marxianus DSM 7238, and Cyberlindnera (C.) jadinii DSM 2361 and DSM 70,163, cultivated on halophyte hydrolysates. In addition, newly isolated strains from halophyte biomass, Lactiplantibacillus (Lpl.) plantarum, Levilactobacillus (Lev.) brevis, and Pichia (P.) fermentans, were bioprospected for their probiotic potential and robustness against relevant environmental stressors. Strains were assessed for viability, tolerance to NaCl and phenol, survival under simulated gastrointestinal condi tions, cell surface properties (hydrophobicity, autoaggregation, biofilm formation), antioxidant activity, and antimicrobial effects. Results revealed great variability among tested strains. Lpl. plantarum and L. lactis demonstrated high salt (> 70% survivability at 5% NaCl) and phenol tolerance (> 80% survivability at 0.3% phenol), strong survivability in simulated digestion (up to ~ 30% survival for Lpl. plantarum and < 10% for L. lactis), and notable antimicrobial activity against up to three tested pathogens, as well as antioxidant activity (up to ~ 55% DPPH scavenging). K. marxianus exhibited high stress tolerance, enhanced antioxidant capacity (up to ~ 70% DPPH scavenging), high survivability after simulated digestion (> 100%), and inhibition of several pathogens. Conversely, B. coagulans showed broad antibiotic resistance, low gastrointestinal survivability (< 5%), and poor growth in halophyte-based media. Overall, Lpl. plantarum and K. marxianus emerged as the most promising candidates for probiotic development and biotechnological applications. These findings highlight halophyte biomass as a selective substrate that favors resilient strains with desirable probiotic traits, supporting its potential in developing sustainable, plant-based fermentation systems. potential probiotics yeast lactic acid bacteria bioprospecting halophytes mikrobiologija probiotiki halofiti substrat in vitro karakterizacija true false true Angleški jezik Slovenski jezik Članek v reviji 2026-04-15 14:06:48 2026-04-15 14:07:02 2026-04-16 04:45:30 0000-00-00 00:00:00 2026 0 0 Str. 455–474 Vol. 29 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2026-03-09 579 1618-1905 10.1007/s10123-026-00786-4 271155203 RAZ_Rudnyckyj_Stanislav_2026.pdf RAZ_Rudnyckyj_Stanislav_2026.pdf 1 F1B2C0C278F76E915F2BDD6E5EE2712C 04ba8de9923071a124abe1b6f0d5e13e880fb4dbf7552a2ab68b17ac5778179a 7209c531-38c3-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=232483 https://link.springer.com/article/10.1007/s10123-026-00786-4 1 6703f81e-38c3-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=232482 Biotehniška fakulteta 0 0 0