20.500.12556/RUL-181666 Effect of archaeal lipids from A. pernix K1 on the in vitro stability and active loading efficiency of liposomes composed of sphingomyelin and cholesterol Archaeal lipids offer a useful strategy for improving liposomal systems because of their inherent stability under extreme conditions. incorporating lipids from Aeropyrum pernix K1 allowed us to optimize sphingomyelin-cholesterol formulation. these archaeal lipids enabled complete recovery of liposome morphology after destabilization with 4 mM ca2+ followed by eDta, indicating that membrane integrity is maintained in the presence of calcium. this is noteworthy given the anionic nature of the archaeal lipids, which in this case provide colloidal stability without increasing sensitivity to ca2+. the addition of 2 mol% mol archaeal lipids also supported high encapsulation efficiency of 95% for active vincristine loading and performed better than the binary mixture when vesicles were prepared by extrusion without cosolvents. Furthermore, reducing the archaeal lipid fraction preserved biological stability provided by sphingomyelin-cholesterol, as confirmed by flow cytometry and fluorescence microscopy, whereas pure archaeosomes composed entirely of archaeal lipids have been reported to show poor stability in biological systems. archaea lipids liposomes drug delivery systems stability vincristine Aeropyrum pernix arheje biološke membrane dostavni sistemi fizikalno-kemijska stabilnost fluidnost membran fosfolipidi holesterol kalcein kapsulacija liposomi sfingomielin stabilnost in vitro vinkristin true false true Angleški jezik Slovenski jezik Članek v reviji 2026-04-10 16:41:50 2026-04-10 16:41:55 2026-04-13 04:41:59 0000-00-00 00:00:00 2026 0 0 Str. [v tisku, 1-15 str.] 2026 0000-00-00 Zaloznikova VTisku NiDoloceno 0000-00-00 0000-00-00 2026-01-01 620.3:577.352:582.233 0898-2104 10.1080/08982104.2026.2619597 268189443 https://www.tandfonline.com/doi/full/10.1080/08982104.2026.2619597 1 36e61d35-34eb-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=232329 RAZ_Kejzar_Jan_2026.pdf RAZ_Kejzar_Jan_2026.pdf 1 222A4C4EA0D303A819643E9A4BA00EA1 f08258b60e72744bd2ebc99917871cd10833c88e8d16e478c52c71bfc7e5fab9 84a8f660-34eb-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=232330 Biotehniška fakulteta 0 0 0