20.500.12556/RUL-181484 Pharmacokinetics of bleomycin in dogs treated with electrochemotherapy Bleomycin (BLM) is a cytotoxic antibiotic used in veterinary oncology, primarily in electrochemotherapy (ECT), a local ablative therapy where electric pulses increase drug uptake in tumours. BLM is administered intravenously or intratumourally, with the standard intravenous dose for dogs being 15,000 IU/m², followed by electric pulses 8–10 minutes later. This protocol is derived from human oncology and lacks extensive pharmacological data in dogs. We studied BLM pharmacokinetics in 29 dogs with various tumours treated with intravenous BLM and ECT between 2017 and 2023. Samples were collected from serum of 15 dogs, serum and tumours of 8 dogs, and tumours of 6 dogs. The mean volume of distribution (Vd) of BLM was 224.5 ± 75.02 ml/kg, clearance (CL) was 7.04 ± 2.05 ml/kg/min, and area under the curve (AUC) was 65.87 ± 2.11 µg·min/l. The half-life (t₁/₂) of BLM in dogs was 22.03 ± 0.88 minutes. No significant difference was found in tumour BLM concentrations between 8 minutes post-administration and 2 minutes after pulse completion. These results support the recommended 8–28-minute window for applying electric pulses following intravenous BLM administration and may indicate no need for dose adjustments based on body weight or age. bleomycin pharmacokinetics electrochemotheapy dogs electroporation serum tumours veterinary bleomicin farmakokinetika elektrokemoterapija psi elektroporacija serum tumorji veterinarska true false true Angleški jezik Slovenski jezik Članek v reviji 2026-04-09 04:43:47 2026-04-09 11:43:14 2026-04-10 04:38:51 0000-00-00 00:00:00 2026 0 0 13 str. iss. 1, art. 2648249 Vol. 46 2026 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 615.033:615.277.3:636.7:615.849 1875-5941 10.1080/01652176.2026.2648249 273830403 Pharmacokinetics_of_bleomycin_in_dogs_treated_with_electrochemotherapy.pdf Pharmacokinetics_of_bleomycin_in_dogs_treated_with_electrochemotherapy.pdf 1 56867C485861A94CB7F42FDD60E29FD1 02949c6d917bbbb4a6cf931323af469bc1cb7212a79939257e2bf1d5c7bbce6a 48f8aa01-33f8-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=232031 https://www.tandfonline.com/doi/full/10.1080/01652176.2026.2648249 1 beda115b-33bd-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=231995 Veterinarska fakulteta Medicinska fakulteta 0 0 0