20.500.12556/RUL-181320 In vitro caecal fermentation and volatile fatty acids production from sugars in rabbits The aim of this study was to evaluate the fermentability of selected sugar substrates using an in vitro gas production test and volatile fatty acid (VFA) analysis with a caecum inoculum from rabbits. The incubated substrates are glucose, fructose, sucrose, molasses and Molkolac® (high lactose content). In addition to determining the gas production of selected substrates, the VFA concentration was also determined after 8 h of incubation in the inoculum prepared from the caecal content of the rabbit. The gas production kinetics parameters were estimated using the Gompertz model and the maximum fermentation rate (MFR), time of maximum fermentation rate (TMFR), lag phase (Lag), amount of gas produced within 8 h (GAS8), maximum fermentation rate within 8 hours (MFR8), total amount of VFA, proportions of each VFA and the ratio between each VFA were determined. Sucrose and molasses yielded the highest gas and VFA production, with molasses showing a slower but intense early fermentation phase. Molkolac exhibited the lowest fermentability. Fermentation of sucrose and glucose was associated with a higher butyrate content, while molasses and Molkolac led to a higher acetate content. Although monosaccharides are normally absorbed in the small intestine, the results of this study confirm that if some sugars, particularly glucose, sucrose, and to a lesser extent fructose, reach the rabbit caecum, they can undergo intense fermentation comparable only to that of sugar beet pulp. In contrast, molasses and lactose (Molkolac) showed lower fermentation efficiency (lower total gas production), with molasses still making a notable contribution due to its rapid onset of fermentation, while lactose had minimal effects. carbohydrates sugars in vitro fermentation caecal inoculum rabbits kunci ogljikovi hidrati sladkorji slepo črevo inokulum in vitro fermentacija hlapne maščobne kisline true false true Angleški jezik Slovenski jezik Članek v reviji 2026-04-01 08:44:53 2026-04-01 08:45:01 2026-04-02 04:25:56 0000-00-00 00:00:00 2026 0 0 Str. 65-73 no. 1 Vol. 34 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2026-03-31 636.92 1989-8886 10.4995/wrs.2026.24114 273807363 RAZ_Rezar_Vida_2026.pdf RAZ_Rezar_Vida_2026.pdf 1 2D2CD29312F79FA17D38EB34609BE81F ed522f0966e9940fd491b0ea849897a648a07554ef5edd5d25d3e96db609edab 3709a57e-2d96-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=231682 https://polipapers.upv.es/index.php/wrs/article/view/24114/18239 1 272d9df6-2d96-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=231681 Biotehniška fakulteta 0 0 0