20.500.12556/RUL-181218 Zelena sinteza srebrovih nanodelcev z ekstraktom kombuče za protibakterijsko delovanje svilene tkanine Kombucha extract-mediated green synthesis of silver nanoparticles for antibacterial activity of silk fabric V magistrskem delu je bil raziskan postopek in situ sinteze srebrovih nanodelcev (Ag ND) na svili z uporabo kombuče, fermentirane pijače, pripravljene s pomočjo simbiotske kulture bakterij in kvasovk (SCOBY), kot okolju prijaznega redukcijskega in stabilizacijskega sredstva. Sinteza je bila izvedena na dva načina: pri prvem so bila vlakna najprej obdelana v kombuči in nato v 0,2 mM raztopini AgNO3, pri drugem pa v obratnem zaporedju. Čas sinteze je bil ena, dve ali štiri ure. Analize morfoloških, kemijskih in optičnih lastnosti so pokazale, da fermentiran čaj kombuče učinkovito deluje kot reducent in stabilizator pri zeleni in situ sintezi Ag ND na svili. Zaporedje reagentov in čas sinteze sta vplivala na kemijske interakcije med vlakni in nanodelci ter na optične značilnosti, medtem ko so bile razlike v vsebnosti srebra med vzorci majhne in statistično nepomembne. Fotoluminiscenčna analiza je pokazala emisijo v modro-vijoličnem območju, značilno za majhne Ag ND v območju nanoklastrov, medtem ko so spektrofotometrične meritve dodatno potrdile spremembe barvnih parametrov in pojav izrazitega vrha površinske plazmonske resonance (SPR, angl. surface plasmon resonance) pri ~380 nm, najopaznejšega po dveh urah sinteze. Funkcionalizirani vzorci svile so izkazovali popolno inhibicijo rasti E. coli in visoko učinkovitost proti S. aureus. Na podlagi rezultatov protibakterijske učinkovitosti je bil kot optimalen izbran prvi način zaporedja reagentov, torej najprej obdelava v fermentiranem čaju kombuče, ki mu je sledil dodatek raztopine AgNO3, s časom sinteze dve uri. Ta kombinacija je zagotovila ohranitev več kot 90 % inhibicije rasti E. coli tudi po desetih ciklih pranja. Rezultati potrjujejo velik potencial uporabe fermentiranega čaja kombuče za trajnostno funkcionalizacijo tekstilij, saj omogoča sintezo Ag ND z minimalno uporabo kemikalij, z zanesljivim in procesno prilagodljivim postopkom, primernim tudi za industrijsko uporabo. The master thesis investigated the in situ synthesis of silver nanoparticles (Ag NPs) on silk using kombucha, a fermented beverage prepared with a symbiotic culture of bacteria and yeast (SCOBY), as an eco-friendly reducing and stabilizing agent. The synthesis was carried out in two ways: In the first approach, the silk was first treated with fermented kombucha tea and then with a 0.2 mM AgNO3 solution, while in the second approach, the sequence was reversed. The synthesis time was 1, 2 or 4 hours. The analyses of morphological, chemical and optical properties showed that kombucha was effective as a reducing and stabilising agent for the green in situ synthesis of Ag NPs on silk. The reagent sequence and the synthesis time influenced the chemical interactions between the fibres and the Ag NPs as well as the optical properties, while the differences in silver content between the samples were small and statistically insignificant. Photoluminescence analysis revealed emission in the blue–violet region, characteristic of small Ag NPs in the nanocluster size range, while spectrophotometric measurements further confirmed changes in colour parameters and the appearance of a pronounced surface plasmon resonance (SPR) peak at ~380 nm, which was most prominent after two hours of synthesis. The functionalized silk samples showed complete inhibition of E. coli growth and high efficacy against S. aureus. Based on the results of antibacterial performance, the first reagent sequence was selected as optimal — treatment with kombucha followed by the addition of AgNO3 solution, with a synthesis time of 2 hours. This combination maintained over 90% inhibition of E. coli growth even after ten wash cycles. The results confirm the great potential of using kombucha for the sustainable functionalisation of textiles, as it enables the synthesis of Ag NPs with minimal use of chemical reagents and is a reliable and process-adaptable method suitable for industrial applications. svila srebrovi nanodelci (Ag ND) zelena in situ sinteza kombuča protibakterijsko delovanje pralna obstojnost silk silver nanoparticles (Ag NPs) green in situ synthesis kombucha antibacterial activity washing durability true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2026-03-27 09:00:53 2026-03-27 09:00:58 2026-03-30 04:27:13 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 113495 28907.pdf 28907.pdf 1 191836499D03B1E075759DCC1DCA0F62 14e3d61377038ac4729e8c02be8d959333d73d3b3ed88d42a1a5b2df47a9dd00 f3f552ea-29b2-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=231445 Naravoslovnotehniška fakulteta 0 0 0