20.500.12556/RUL-181010 Proučevanje fenolnih spojin in alergenih proteinov v izbranih sortah jabolk Study of phenolic compounds and allergenic proteins in selected apple varieties Proučevali smo povezavo med vsebnostjo fenolnih spojin in alergenih proteinov Mal d 1 in Mal d 3 v štirih sortah jabolk (‘Carjevič’, ‘Gala’, ‘Topaz’ in ‘Zlati delišes’) tako v mesu kot v kožici plodov. Namen raziskave je bil ugotoviti, ali obstajajo razlike med sortami in tkivoma (meso/kožica) v vsebnosti fenolov in proteinov ter morebitne povezave med fenolnim profilom in izraženostjo alergenov. Za kvantifikacijo fenolov smo uporabili visoko ločljivostno tekočinsko kromatografijo (HPLC), za določitev alergenih proteinov pa Western blot analizo s kvantifikacijo v programu ImageJ (verzija 1.54p, ZDA). Ugotovili smo, da so bile razlike v vsebnosti fenolov med sortami statistično značilne, pri čemer sta ‘Topaz’ in ‘Gala’ izstopali po največji vsebnosti flavanolov v kožici, ‘Gala’ pa je imela tudi značilno največjo vsebnost antocianinov. Mal d 1 je bil prisoten v vseh sortah, njegovo največjo vsebnost smo izmerili v mesu jabolk ‘Carjevič’ in v kožici ‘Topaz’, medtem ko je bil Mal d 3 zaznan le v kožici. Korelacijska analiza je pokazala močno negativno povezavo med Mal d 1 in hidroksicimetnimi kislinami ter pozitivno korelacijo z dihidrohalkoni in flavanoli. Ugotovitve potrjujejo, da sta fenolni profil in alergenost jabolk močno odvisna od sorte in dela ploda. Rezultati prispevajo k boljšemu razumevanju povezav v sintezi fenolnih spojin in alergenih proteinov v jabolku ter omogočajo usmerjeno selekcijo sort z nižjo alergenostjo ob ohranjanju hranilne vrednosti. We studied the relationship between the content of phenolic compounds and the allergenic proteins Mal d 1 and Mal d 3 in four apple varieties (‘Crown Prince Rudolph’, ‘Gala’, ‘Topaz’, and ‘Golden Delicious’), analysing both the flesh and peel of the fruit. This study aimed to determine whether differences in phenolic and protein contents exist among cultivars and tissues (flesh/peel), and to identify a potential correlation between the phenolic profile and the expression of allergenic proteins. Phenolic compounds were quantified using high-performance liquid chromatography (HPLC), while allergenic proteins were determined using Western blot analysis with subsequent quantification in the ImageJ program (version 1.54p, USA). We found statistically significant differences in phenolic compounds among cultivars. ‘Topaz’ and ‘Gala’ showed the highest flavanol content in the peel, while ‘Gala’ also showed the highest content of anthocyanins. Mal d 1 protein was detected in all cultivars, with the highest levels measured in the flesh of ‘Crown Prince Rudolph’ and in the peel of ‘Topaz’, whereas Mal d 3 protein was detected only in the peel. Correlation analysis showed a strong negative association between Mal d 1 and hydroxycinnamic acids, and a positive correlation with dihydrochalcones and flavanols. The findings indicate that both the phenolic profile and allergenic potential of apples are strongly dependent on cultivar and fruit tissue. The results contribute to a better understanding of the interaction between the biosynthesis of phenolic compounds and allergenic proteins in apple and support targeted selection of cultivars with reduced allergenicity while preserving nutritional value. jabolka ‘Topaz’ ‘Gala’ ‘Zlati delišes’ ‘Carjevič’ fenolne spojine alergeni proteini Mal d 1 Mal d 3 HPLC Western blot korelacijska analiza apples ‘Topaz’ ‘Gala’ ‘Golden Delicious’ ‘Crown Prince Rudolph’ phenolic compounds allergenic proteins Mal d 1 Mal d 3 HPLC Western blot correlation analysis true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2026-03-22 07:16:01 2026-03-22 07:16:07 2026-03-23 13:37:01 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 279196 272663043 56806.pdf 56806.pdf 1 253CF2F07826D697C04FA1E577884A30 476ee100e385ae24ed9e979a737f5c607a274780514850c8806fe9d52b4adb56 8402158d-25b6-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=231063 56807.pdf 56807.pdf 1 051B6C9CF6D1FAEE5909628F9E5D783D 25d3881551e28c7f4edb07007d06f1fb3b2c07db300b6fda1d135ac61be058ec 8402de08-25b6-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=231064 56808.pdf 56808.pdf 1 3D2B6E3281A1BDEFCE0E01E4016F4C24 5a61aaeb4dfa471b076a730ea3b0ddfb22bd2f551d2ef69ae8776c2675941579 8404d224-25b6-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=231065 Biotehniška fakulteta 0 0 0