20.500.12556/RUL-180993 Vpliv izbranih antioksidantov na rast in metabolizem bakterije Eubacterium rectale The influence of selected antioxidants on the growth and metabolism of Eubacterium rectale Striktno anaerobne, butirat-producirajoče bakterije črevesne mikrobiote imajo pomembno vlogo pri ohranjanju zdravja gostitelja, vendar je njihov odziv na prehranske antioksidante še slabo raziskan. V tej raziskavi smo preučevali vpliv askorbinske kisline, propil galata, ferulne kisline in α-tokoferola na rast, metabolno aktivnost in membransko sestavo bakterije Eubacterium rectale Do2-077. Bakterijo smo gojili v anaerobnih razmerah v prisotnosti različnih koncentracij antioksidantov ter spremljali rast z merjenjem optične gostote, metabolno aktivnost z analizo plinskih metabolitov (H₂, CO₂) in produkcijo kratkoverižnih maščobnih kislin ter spremembe v maščobnokislinskem profilu membran. Učinki so se razlikovali med vrstami in koncentracijami uporabljenih antioksidantov. Askorbinska kislina je statistično značilno vplivala na rast ter povečala produkcijo H₂ in CO₂. Propil galat je pri nižjih koncentracijah deloval spodbujevalno, pri višjih zaviralno na metabolno aktivnost, medtem ko ferulna kislina ni bistveno vplivala na rast, vendar je spremenila razmerje fermentacijskih plinov. Prisotnost α-tokoferola je bila povezana s spremembo razmerja fermentacijskih produktov, zlasti s povečano tvorbo acetata in propionata. Pri produkciji butirata ter spremembah maščobnokislinskega profila membran med skupinami večinoma nismo ugotovili statistično značilnih razlik, so pa trendi nakazovali, da na sestavo lahko vplivajo askorbinska kislina, ferulna kislina in α-tokoferol. Skupno rezultati kažejo, da lahko antioksidanti vplivajo na presnovno aktivnost potencialnega probiotika nove generacije E. rectale Do2-077, vendar so opaženi učinki omejeni in zahtevajo dodatno potrditev z večjim številom ponovitev. Strictly anaerobic, butyrate-producing bacteria in the gut microbiota play an important role in maintaining gut health, but their response to dietary antioxidants remains poorly understood. In this study, we investigated the effect of ascorbic acid, propyl gallate, ferulic acid, and α-tocopherol on the growth, metabolic activity, and membrane composition of Eubacterium rectale Do2-077. The bacteria were cultured under anaerobic conditions in the presence of different concentrations of antioxidants, and growth was monitored by measuring optical density, metabolic activity by analyzing gas metabolites (H₂, CO₂) and production of short-chain fatty acids, as well as changes in membrane fatty acid profile. The effects varied depending on the type and concentration of antioxidants used. Ascorbic acid had a statistically significant effect on growth and increased H₂ and CO₂ production. Propyl gallate had a stimulating effect at lower concentrations and an inhibitory effect on metabolic activity at higher concentrations, while ferulic acid did not significantly affect growth but altered the fermentation gases ratio. The presence of α-tocopherol was associated with a change in the ratio of fermentation products, particularly with increased formation of acetate and propionate. Changes in the production of butyrate and membrane fatty acid profile were not statistically confirmed, but trends indicated that ascorbic acid, ferulic acid and α-tocopherol may have influenced the membrane composition. Collectively, the results suggest that antioxidants may influence the metabolic activity of the potential next generation probiotic strain E. rectale Do2-077, but the observed effects are limited and require further confirmation with a larger number of replicates. Eubacterium rectale antioksidanti črevesna mikrobiota anaerobne bakterije kratkoverižne maščobne kisline metabolna aktivnost probiotiki nove generacije Eubacterium rectale antioxidants gut microbiota anaerobic bacteria short-chain fatty acids metabolic activity next generation probiotics true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2026-03-21 07:15:17 2026-03-21 07:15:51 2026-04-08 13:51:24 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 279170 272575747 56995.pdf 56995.pdf 1 037FC451E153043B103821323750B141 e6fc9b0269a8389b73beba9817960c451605ab635d1e165e89c84fce261e4f78 5b840bb9-24ed-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=231043 Biotehniška fakulteta 0 0 0