20.500.12556/RUL-180812 Vpliv izlužkov zelenega betona z dodanim bioogljem na različne organizme The impact of biochar-based green concrete leachates on different organisms Gradbeništvo je eden največjih porabnikov naravnih virov in povzročiteljev emisij toplogrednih plinov, predvsem zaradi proizvodnje cementa. Za zmanjšanje okoljskih obremenitev se razvija zeleni beton, pri katerem se del agregata ali cementa nadomešča z odpadnimi materiali, med katerimi se je biooglje izkazalo za posebej obetavno pri nadomeščanju določenega deleža cementa v betonu. Tak beton praviloma izkazuje izboljšane mehanske, reološke in trajnostne lastnosti, kljub prednostim pa ostaja ključno vprašanje, kako materiali z dodatkom biooglja pod vplivom različnih dejavnikov učinkujejo na okolje in posledično na organizme. Namen magistrskega dela je bil raziskati vpliv izlužkov klasičnega in zelenega betona z bioogljem na štiri različne organizme: malo vodno lečo (Lemna minor), vodno bolho (Daphnia magna), čebulo (Allium cepa) in belo gorjušico (Sinapis alba). Pri mali vodni leči smo opazovali rast členov in korenin ter produkcijo fotosintetskih pigmentov, pri vodnih bolhah mobilnost, pri čebuli in beli gorjušici pa dolžino korenin. Rezultati testov z malo vodno lečo so pokazali, da izlužki zelenega betona z višjo koncentracijo močneje zavirajo rast členov, pri najnižji koncentraciji pa so bolj izrazito prispevali k boljši rasti v primerjavi s klasičnim betonom. Pri rasti korenin in skupnem klorofilu se je zeleni beton izkazal za manj problematičnega, pri čemer tudi klasični beton ni dosegel toksičnih vrednosti. Podoben vzorec nizke obremenitve okolja se je potrdil pri vodnih bolhah, ki so pri vseh koncentracijah obeh vrst betona izkazale zanemarljivo nizke inhibicije. Pri beli gorjušici smo ugotovili, da se je dolžina korenin ob zniževanju koncentracije zelenega betona povečevala, medtem ko je bil pri klasičnem betonu opažen obraten trend; kljub temu tudi tukaj inhibicije niso presegale 50 %. Čebula je izkazala najmanjši vpliv, saj je pri vseh vzorcih reagirala stimulativno, najizraziteje v prisotnosti zelenega betona. Kljub razlikam v odzivih posameznih testnih organizmov rezultati kažejo, da je zeleni beton manj toksičen od klasičnega, izjema je bila le rast členov pri mali vodni leči. Izlužki zelenega in klasičnega betona so se izkazali kot manj toksični za kopenske (čebulo in belo gorjušico) kot za vodne organizme (vodne bolhe in malo vodno lečo). Construction is one of the largest consumers of natural resources and a major contributor to greenhouse gas emissions, primarily due to cement production. To reduce environmental impacts, green concrete is being developed, in which part of the aggregate or cement is replaced with waste materials, among which biochar has proven particularly promising for substituting a proportion of cement in concrete. Such concrete typically exhibits improved mechanical, rheological, and durability properties; however, despite these advantages, a key question remains regarding how materials with added biochar affect the environment and, consequently, organisms under various conditions. The aim of this master’s thesis was to investigate the effects of leachates from conventional and green concrete containing biochar on four different organisms: duckweed (Lemna minor), water flea (Daphnia magna), onion (Allium cepa), and white mustard (Sinapis alba). For duckweed, frond and root growth as well as the production of photosynthetic pigments were observed; for water fleas, mobility was monitored; for onion and white mustard, root length was measured. Results from duckweed tests showed that leachates from green concrete at higher concentrations more strongly inhibited frond growth, whereas at the lowest concentration they contributed more markedly to enhanced growth compared to conventional concrete. Regarding root growth and total chlorophyll content, green concrete proved to be less problematic, while conventional concrete did not reach toxic levels. A similar pattern of low environmental impact was confirmed for water fleas, which exhibited negligibly low inhibition at all concentrations of both types of concrete. For white mustard, root length increased with decreasing concentrations of green concrete, while an opposite trend was observed for conventional concrete; nonetheless, inhibition did not exceed 50 % in either case. Onion showed the lowest level of impact, as it responded stimulatively in all samples, with the strongest response observed in the presence of green concrete. Despite differences in the responses of individual test organisms, the results indicate that green concrete is less toxic than conventional concrete, except for frond growth in duckweed. Leachates from both green and conventional concrete proved less toxic to terrestrial organisms (onion and white mustard) than to aquatic organisms (water fleas and duckweed). biooglje cement ekotoksikološki testi izlužki zeleni beton biochar cement ecotoxicological tests green concrete leachates true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2026-03-17 09:35:02 2026-03-17 09:35:18 2026-03-27 11:15:24 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 25487 273337603 Tratar_Leja_-_Vpliv_izluzkov_zelenega_betona_z_dodanim_bioogljem_na_razlicne_organizme.pdf Tratar_Leja_-_Vpliv_izluzkov_zelenega_betona_z_dodanim_bioogljem_na_razlicne_organizme.pdf 1 EDBA32B1EC60A6DAA896E416F7B4DB58 8b4ef6fee9aca398e5bc4e77bb0772c07bd33ed55504a574b4b1c51129b79cb8 2bcf09a9-21dc-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=230667 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0