20.500.12556/RUL-180646 Transcriptomic insight into early response of apple infected with Penicillium expansum exposed to blue LED light The aim of the study was to investigate the early response of apple fruit infected with Penicillium expansum (P. expansum) to blue light-emitting diode (LED) light (BLL) irradition. To focus our study on the interaction between apple fruit, the pathogen, and BLL, the effect of BLL was also studied on apples without P. expansum and P. expansum grown on malt extract agar (MEA). Transcriptome analysis revealed that the most pronounced responses among biological processes were observed in inoculated apples under BLL. The upregulated processes included water transport, response to heat, and response to high light intensity. The defence response of apples was enhanced by the upregulation of thaumatin-like proteins and caffeic acid 3-O-methyltransferase, while the cellular response to phosphate deficiency and the regulation of multicellular organism development were downregulated. In P. expansum grown on apples under BLL, transcriptome analysis revealed downregulation of genes related to signalling, response to organic compounds, and regulation of metabolic and biosynthetic processes, while genes involved in the biosynthesis of secondary metabolites were upregulated. In addition, the expression of patulin cluster genes was predominantly downregulated in P. expansum. The significant upregulation of genes related to cryptochrome inhibition, defence response, and caffeic acid metabolism in apples under BLL, together with the reduced virulence of P. expansum, contributes to the inhibition of fungal growth. apple fruit Penicillium expansum storage physical treatment blue LED light RNA-Seq plesni Penicillium expansum jabolka skladiščenje jabolk obsevanje modra LED-svetloba true false true Angleški jezik Slovenski jezik Članek v reviji 2026-03-12 14:50:12 2026-03-12 14:50:20 2026-03-13 17:10:41 0000-00-00 00:00:00 2026 0 0 18 str. issue 2, art. 246 Vol. 16 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2026-01-01 664.8.03:634.11:579.67 2073-4395 10.3390/agronomy16020246 265522435 RAZ_Mahnic_Nik_2026.pdf RAZ_Mahnic_Nik_2026.pdf 1 64A4D925DF494EA67C7B4BAA3448BDBB 1b573dc12af899ace5672b1fe6fa236a5eb035f19f1dd3fff80f78ca23f5cde7 a49b67fb-1e1b-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=230244 https://www.mdpi.com/2073-4395/16/2/246 1 66ff4241-1e1a-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=230243 Biotehniška fakulteta Fakulteta za elektrotehniko 0 0 0