20.500.12556/RUL-180564 In vitro bioaccessibility of lutein diesters in relation to their chemical structure This study investigated the bioaccessibility of ten synthetic lutein diesters using the in vitro INFOGEST human digestion model, enabling comparative evaluation of their digestive behavior under controlled food-matrix conditions. We revealed that lutein ester chain length was the primary determinant of bioaccessibility, while branching influenced the extent of hydrolysis. Linear short-chain diesters demonstrated superior bioaccessibility (6.7–18.9%). Branched and bulky derivatives, surprisingly, achieved comparable bioaccessibility (6.6–8.3%) despite high hydrolytic resistance, suggesting mechanisms beyond simple ester cleavage. Conversely, long-chain lutein diesters (> C10), prevalent in commercial supplements, exhibited poor micellization, limited hydrolysis, and consequently very low bioaccessibility (<3%). Finally, two pharmaceutical formulations of the lead lutein diester, lutein di(2,2-dimethylpropanoate), were developed: a sunflower oil formulation and a liquisolid formulation utilizing a porous aluminometasilicate carrier. The sunflower oil formulation enabled significantly higher pigment stability and bioaccessibility, highlighting its superior potential for effective lutein delivery in novel food and pharmaceutical applications. lutein diesters INFOGEST in vitro digestion bioaccessibility hydrolysis efficiency chemical structure structure-property relationship luteinski diestri INFOGEST in vitro prebava biološka dostopnost učinkovitost hidrolize kemijska struktura razmerje med strukturo in lastnostmi prebavne motnje true false true Angleški jezik Slovenski jezik Članek v reviji 2026-03-11 12:03:44 2026-03-11 12:03:54 2026-03-12 04:44:53 0000-00-00 00:00:00 2026 0 0 12 str. art. 148572 Vol. 509 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 542:616.3-008 1873-7072 10.1016/j.foodchem.2026.148572 271138307 1-s2.0-S0308814626007302-main.pdf 1-s2.0-S0308814626007302-main.pdf 1 4B0450F5866E6504D4EEDBCCD1BFA2CA 9c81ef1a9ad86a8175991ad0dd59f191881f89015a9cf83d3a2b90c3709e07f1 b7509b2c-1d3a-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=230027 https://www.sciencedirect.com/science/article/pii/S0308814626007302 1 025c0c91-1d3a-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=230026 Fakulteta za farmacijo 0 0 0