20.500.12556/RUL-180554 ACTN3, IL-6 and PPARA gene polymorphisms and anaerobic performance in volleyball players Polimorfizmi genov ACTN3, IL-6 in PPARA ter anaerobna zmogljivost pri odbojkarjih The aim of this study is to determine the relationship between ACTN3 rs1815739, IL-6 rs1800795, and PPARA rs4253778 gene polymorphisms and anaerobic test parameters among volleyball players. A total of 22 volleyball players voluntarily participated in the study. DNA isolation from buccal epithelial cell samples were performed using a PureLink DNA isolation kit, following the manufacturer's instructions. The genotyping of ACTN3 rs1815739, IL-6 rs1800795, and PPARA rs4253778 gene polymorphisms were determined using the real-time polymerase chain reaction technique. Statistical analysis of the test results was performed using the SPSS 23 software package. Homogeneity of variance was assessed with the Levene test, and normal distribution was evaluated using the Shapiro-Wilk test. The One-Way ANOVA test was applied for the analysis of all parameters, and significance was set at p < 0.05. When the data were evaluated, no statistically significant differences were observed between PPARA rs4253778 and ACTN3 rs1815739 gene polymorphisms and anaerobic performance parameters (p>0.05). Statistically significant differences were observed between the IL-6 rs1800795 gene polymorphism and anaerobic performance parameters of back strength (p> 0.05). In conclusion, in volleyball, PPARA rs4253778 and ACTN3 rs1815739 gene polymorphisms did not cause differentiation in anaerobic performance parameters. However, IL-6 polymorphism caused differentiation only in the back strength parameter. Additionally, the good performance of athletes with different prevalent level of genotypes in the studied performance criteria does not fully reveal which performance outcomes are influenced by the gene or genotype distributions. It is understood that further studies are needed to better determine the gene-performance relationship. Cilj te študije je bil ugotoviti povezavo med polimorfizmi genov ACTN3 rs1815739, IL-6 rs1800795 in PPARA rs4253778 ter parametri anaerobnih testov pri odbojkarjih. V raziskavi je prostovoljno sodelovalo 22 odbojkarjev. Izolacija DNK iz vzorcev bukalnih epitelijskih celic je bila izvedena s pomočjo kompleta PureLink za izolacijo DNK, v skladu z navodili proizvajalca. Genotipizacija polimorfizmov genov ACTN3 rs1815739, IL-6 rs1800795 gene in PPARA rs4253778 je bila določena z uporabo tehnike verižne reakcije s polimerazo v realnem času (real-time PCR). Statistična analiza rezultatov je bila izvedena s programskim paketom SPSS 23. Homogenost varianc je bila preverjena z Levenovim testom, normalnost porazdelitve pa z uporabo Shapiro–Wilkovega testa. Za analizo vseh parametrov je bil uporabljen enosmerni ANOVA test, statistična značilnost pa je bila določena pri p < 0,05. Rezultati so pokazali, da med polimorfizmoma genov PPARA rs4253778 in ACTN3 rs1815739 ter parametri anaerobne zmogljivosti niso bile ugotovljene statistično značilne razlike (p > 0,05). Statistično značilne razlike so bile ugotovljene med polimorfizmom gena IL-6 rs1800795 in parametrom anaerobne zmogljivosti hrbtne moči (p < 0,05). Torej, pri odbojki polimorfizma genov PPARA rs4253778 in ACTN3 rs1815739 nista povzročila razlik v parametrih anaerobne zmogljivosti. Vendar je polimorfizem gena IL-6 vplival na razlikovanje zgolj pri parametru hrbtne moči. Poleg tega dobra zmogljivost športnikov z različno zastopanimi genotipi pri proučevanih merilih ne razkriva popolnoma, katere dejavnike zmogljivosti dejansko določajo geni oziroma njihova porazdelitev. Jasno je, da so potrebne nadaljnje raziskave za natančnejšo opredelitev odnosa med geni in zmogljivostjo. sport polymorphism gene volleyball šport polimorfizem gen odbojka true false true Angleški jezik Slovenski jezik Podatek o licenci CC BY-SA 4.0 je naveden na pristajalni strani članka (glej zgoraj izvorni URL). (Datum opombe: 12. 3. 2026) Članek v reviji 2026-03-11 09:01:22 2026-03-11 09:01:22 2026-03-26 15:02:45 0000-00-00 00:00:00 2025 0 0 Str. 182-203 no. 3 Vol. 31 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 796.325 1318-2269 10.52165/kinsi.31.3.182-203 265809923 10_On_urejeno_novo_180554.pdf 10_On_urejeno_novo_180554.pdf 1 E366CC9CF46CF04250370670AFC9143C 2b919cd5ce31e3188658352d9201771f7e3d7f46785e416b713bede584cdbf95 fb96c501-1d44-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=230079 https://journals.uni-lj.si/kinsi/article/view/24337 0 110ba74e-1d20-11f1-a1ba-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=230004 Fakulteta za šport 0 0 0