20.500.12556/RUL-180357 From breeding to practise effects of sowing date and pruning system on flower yield and cannabinoid composition in four Slovenian feminized industrial hemp breeding lines Od žlahtnjenja do uporabe: vpliv datuma setve in rezi na pridelek cvetov in vsebnost kanabinoidov v štirih slovenskih žlahtnjenih linijah feminizirane industrijske konoplje our Slovenian feminized industrial hemp (Cannabis sativa L.) breeding lines (G8c, G10c, MERc, CHc), developed within the high‑CBD breeding program at the Biotechnical Faculty, University of Ljubljana, were evaluated in 2024 field trials at Ljubljana and Krško. The effects of sowing date (direct field seed sowing vs. seedlings transplantation to the field, both at density 8,333 plants per ha) and apical pruning (topping) on growth (plant height), flower yield, CBD, and Δ⁹ THC content were assessed. Early- season waterlogging in Ljubljana (heavy soil) caused severe negative effects on plant establishment and growth. Sowing date acted oppositely across sites: direct sowing outperformed transplanting in Krško (medium heavy soil), whereas transplanting increased flower yield in Ljubljana. Pruning reduced plant height but did not change flower yield or cannabinoid concentrations. CBD was primarily genotype dependent, with MERc the highest at both sites (5.02-6.66 %). THC ranged 0.12–0.21 % across treatments. Significant breeding line × sowing date interactions occurred for plant height and flower yield. This study provides the first applied evaluation of Slovenian feminized industrial hemp breeding lines and indicates priorities for subsequent breeding efforts. However, the results represent one year of field data .To draw robust conclusions, multi-year field trials will be required. industrial hemp feminized hemp high-CBD genotype sowing date transplanting pruning CBD THC industrijska konoplja feminizirana konoplja CBD konoplja datum setve presajanje rez CBD THC true false true Angleški jezik Slovenski jezik Članek v reviji 2026-03-06 11:42:39 2026-03-06 11:42:46 2026-03-07 04:40:09 0000-00-00 00:00:00 2026 0 0 15 str. no. 1 Vol. 122 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 633 1854-1941 10.14720/aas.2026.122.1.26115 270751747 Flajsman+in+Erzen+(N).pdf Flajsman+in+Erzen+(N).pdf 1 FB988150A0BD3D55D97ABDF549C65408 eed527702dd85d9265399f2c972079baee1c000439c8da2c6608ba2512e0e441 07e7bef0-1949-11f1-a1ba-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=229670 https://journals.uni-lj.si/aas/article/view/26115/21080 1 d2fdab64-1948-11f1-a1ba-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=229669 Biotehniška fakulteta Acta agriculturae Slovenica 0 0 0