20.500.12556/RUL-180316 Uporaba srebrovih spojin za indukcijo moških cvetov na ženskih rastlinah navadne konoplje (Cannabis sativa) Use of silver compounds for the induction of male flowers on female plants of common hemp (Cannabis sativa) Indukcija moških cvetov na genetsko ženskih rastlinah navadne konoplje s pomočjo srebrovih spojin je uveljavljena metoda za pridobivanje feminiziranih semen, ki so ključna za homogeno in kakovostno pridelavo kanabinoidov. Srebrove spojine, kot sta srebrov tiosulfat (STS) in koloidno srebro (KS), delujejo kot inhibitorji etilena, s čimer spodbujajo razvoj moških reproduktivnih organov na genetsko ženskih rastlinah. V tej raziskavi smo preučili učinkovitost različnih koncentracij in nanosov STS (0,5 mM, 5 mM, 10 mM, 20 mM) ter KS (30 ppm) pri indukciji moških cvetov na ženskih rastlinah konoplje sorte 'Matterhorn'. V raziskavi smo merili prirast rastlin, odstotek moških cvetov, suho maso rastlin in kalivost pridobljenega peloda. Za proizvodnjo femeniziranega semena konoplje se je kot najprimernejše obravnavanje izkazala enkratna uporaba 20 mM STS zaradi visoke stopnje maskulinizacije (3,64), relativno dobre kalivosti peloda (21,72 %) in najmanjšega negativnega vpliva na vitalnost rastlin. Tudi trikratni nanos 10 mM STS je bil enako učinkovit pri stopnji maskulinizacije (3,65). Rezultati potrjujejo, da so srebrove spojine učinkovito orodje za manipulacijo spolnega izražanja pri konoplji, vendar je potrebna nadaljnja optimizacija za zmanjšanje fitotoksičnosti in izboljšanje kakovosti peloda. The induction of male flowers on genetically female hemp plants using silver compounds is an established method for producing feminized seeds, which are essential for the uniform and high-quality production of cannabinoids. Silver compounds such as silver thiosulfate (STS) and colloidal silver (CS) act as ethylene inhibitors, promoting the development of male reproductive organs on genetically female plants. In this study, we evaluated the effectiveness of different concentrations and applications of STS (0.5 mM, 5 mM, 10 mM, 20 mM) and colloidal silver (30 ppm) in inducing male flowers on female plants of the ‘Matterhorn’ cannabis strain. We measured plant growth, the percentage of male flowers, plant dry weight, and the germination rate of the pollen obtained. For the production of feminized cannabis seeds, a single application of 20 mM STS was the most suitable treatment, resulting in a high degree of masculinization (3.64), relatively good pollen germination (21.72%), and the least negative impact on plant vitality. Treatment with 10 mM STS applied three times was equally effective in terms of the degree of masculinization (3.65). The results confirm that silver compounds are effective for manipulating sex expression in hemp, but further optimization is needed to reduce phytotoxicity and improve pollen quality. Cannabis sativa indukcija spola srebrove spojine srebrov tiosulfat koloidno srebro feminizirana semena kalivost peloda Cannabis sativa sex induction silver compounds silver thiosulfate colloidal silver feminized seeds pollen germination true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2026-03-06 07:15:07 2026-03-06 07:15:19 2026-03-07 04:40:05 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 279057 270796803 56699.pdf 56699.pdf 1 6698F8B0CC4D4CF3415466F3519593D6 01c68516a80851be3e526c23f104cc0ccf41c292d65412f0b3fe985a526f190e 7ba4504f-1923-11f1-a1ba-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=229586 Biotehniška fakulteta 0 0 0