20.500.12556/RUL-180062 Effect of SPbetaviruses on kin discrimination in Bacillus subtilis Vpliv Spbetavirusov na sorodstveno razlikovanje pri bakteriji Bacillus subtilis In this master's thesis, we investigated the role of lysogenic bacteriophages from the Spbetavirus genus in kin discrimination in the bacterium Bacillus subtilis. We studied how the integration of prophages changes social interactions between strains. The results confirmed that the mere presence of a prophage can shift an interaction from kin to non-kin. By analysing phage release using a confluent plaque assay, we found that the induction of prophages at the boundary line where the swarming lysogens meet is not strongly linked to the kin system. A subsequent cross-immunity test revealed that the phi3T lysogen can lyse all other lysogens, while its lysogen was susceptible to three other phages. This unique position of phi3T is hypothesised to contribute to a more intense conflicts at colony boundaries. Collectively, the evidence indicates that Spbetavirus prophages function as significant genetic modulators of bacterial sociality, with the phi3T phage playing a special role in lysogen interactions. The precise mechanisms by which host interactions influence the switch of prophages to the lytic cycle remain a subject for future research. V tem magistrskem delu smo raziskovali vlogo lizogenih bakteriofagov iz rodu Spbetavirus v sorodstvenem razlikovanju pri bakteriji Bacillus subtilis. Preučevali smo, kako vstavljeni profagi spreminjajo socialne interakcije med gostiteljskimi sevi. Rezultati so potrdili, da lahko prisotnost profaga spremeni interakcijo iz kin v ne-kin. Z analizo sprostitve fagov s konfluentnim testom plakov smo ugotovili, da aktivacija profaga na mestu stika rojev sevov v večini ni povezana s sistemom sorodstvene diskriminacije. Nadaljnji test navzkrižne imunosti je razkril, da je phi3T edini sposoben lizirati vse druge lizogene seve, njegov lizogen pa je bil dovzeten za tri druge raziskane fage. Ta edinstven položaj phi3T verjetno prispeva k intenzivnejšim negativnim interakcijam med rojenjem. Skupaj rezultati kažejo, da fagi Spbetavirus delujejo kot pomembni genetski modulatorji bakterijske socialnosti, pri čemer fag phi3T igra posebno vlogo v interakcijah med lizogeni. Natančni mehanizmi, s katerimi interakcije vplivajo na preklop profagov v litični cikel, ostajajo predmet prihodnjih raziskav. lysogenic bacteriophages kin discrimination prophage induction Bacillus subtilis Spbetavirus lizogeni bakteriofagi sorodstvena diskriminacija indukcija profaga Bacillus subtilis Spbetavirus true false false [G. Cotič] Angleški jezik Slovenski jezik Magistrsko delo/naloga 2026-03-01 07:15:25 2026-03-01 07:15:31 2026-03-02 08:12:52 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 602.3:578.347:601.4:577.21:602.4 279030 270024707 56701.pdf 56701.pdf 1 8104DD6BD22B270C59D5A20474B65B63 983db3ad4671314aeb45d97380778625c525939f331e2a343715419b1b483463 b3124095-1535-11f1-a1ba-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=229078 Biotehniška fakulteta 0 0 0