20.500.12556/RUL-179719 INSL3 expression in Leydig cells is a biomarker for immunocastration in boars transcriptional evidence Background: Study aimed to obtain insights into physiological responses to immunocastration in pubertal boars by evaluating effects of alternative vaccination protocols and identifying a reliable immunocastration biomarker. Objectives: It was hypothesized that the timing of gonadotropin-releasing hormone (GnRH) suppression by immunocastration differentially affects reproductive function, as reflected by testicular histology and expression of selected genes related to testicular function, steroid metabolism, and Leydig cell differentiation and function. Materials and Methods: Effects of three vaccination protocols on antibody titers, testicular histomorphology, and mRNA expression in immunocastrates slaughtered 4, 8, or 12 weeks (IC-4, IC-8, and IC-12, respectively; n = 6 per group) after booster were compared with entire males (EMs; n = 6). Principal component analysis and hierarchical clustering were used to evaluate individual responses and to identify an immunocastration biomarker(s). The selected biomarker was also validated on samples from previous studies. Results: All immunocastrated boars reacted immunologically to vaccination (increased GnRH antibody titer; p < 0.004). There was an increased nucleus-to-cytoplasm ratio in Leydig cells (p < 0.033) and decreased testosterone concentration (p < 0.041) in IC-4 compared with EM, whereas values in IC-8 and IC-12 were intermediate. Hierarchical clustering differentiated immunocastrates with low testosterone (IC-LT; median 0.56 ng/mL) and high testosterone (IC-HT; median 7.04 ng/mL). In IC-LT, expression levels of FSHR and ESR2 were higher than in IC-HT, and those of LHCGH, STAR and INSL3 were lower compared with IC-HT and EM, whereas expression of ESR1 and HSD17β7 was lower in IC-LT and IC-HT compared with EM (fold change > 1.5 and p < 0.05). Conclusion: There were variable responses to immunocastration in pubertal boars subjected to three vaccination protocols. Suppression of testicular function was most pronounced in boars slaughtered 4 weeks after the booster, whereas progressive recovery occurred in some boars 8 and 12 weeks after the booster. Irrespective of vaccination protocol, INSL3 mRNA expression was a reliable immunocastration biomarker. alternatives to castration pigs INSL3 response to immunocastration testicular mRNA expression true false true Angleški jezik Ni določen Članek v reviji 2026-02-21 04:40:38 2026-02-23 11:01:07 2026-03-20 04:36:08 0000-00-00 00:00:00 2026 0 0 Str. 782-795 iss. 3 Vol. 14 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 602 2047-2927 10.1111/andr.70136 254427651 Andrology_-_2025_-_Lukac_-_INSL3_Expression_in_Leydig_Cells_is_a_Biomarker_for_Immunocastration_in_Boars__Transcriptional_(1).pdf Andrology_-_2025_-_Lukac_-_INSL3_Expression_in_Leydig_Cells_is_a_Biomarker_for_Immunocastration_in_Boars__Transcriptional_(1).pdf 1 BAACB7A3EE2F30B876E479A888C4B327 2b1a468715499e9199c4d24d85bc706e43a0ac21141ef5a364f4a6fa718d747b c57e22ef-23a1-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=230904 https://onlinelibrary.wiley.com/doi/10.1111/andr.70136 0 a357e710-23a1-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=230903 Veterinarska fakulteta 0 0 0