20.500.12556/RUL-179552 Comparison of tooth nuclear DNA preservation between cementum and other dental tissues For the genetic identification of skeletal remains, teeth can be used since they contain well-preserved DNA, which is present in various dental tissues, including dental pulp, dentin, and cementum. In forensics, the standard extraction method utilizes the whole tooth and destructive grinding. In our previous study, a highly effective non-destructive method was introduced to extract DNA from tooth cementum, bypassing whole tooth destruction. An analysis was performed on sixty-two canines obtained from adult skeletons from two archaeological cemeteries. After tooth cementum DNA extraction, teeth were stored in the freezer, and later used for destructive DNA extraction, grinding the entire tooth left after non-destructive extraction. The purpose of this study was to compare the preservation of DNA in tooth cementum and other tooth tissues. To extract the DNA from the stored teeth, they were pulverized, and DNA was obtained using the full demineralization method. Real-time PCR was employed to evaluate the quality and quantity of DNA, followed by STR typing. The amount of DNA, degradation rate, and success of STR typing were compared between DNA retrieved from tooth cementum and DNA extracted from the other tooth tissues. The results showed no successful STR typing in most stored teeth, and only five of them produced full or almost full profiles. Comparison to tooth root cementum, where 46 out of 62 teeth generated highly informative STR profiles, indicates that in the aged canines analysed, DNA preservation is strongest in tooth root cementum, while other tooth tissues generally yield little or no recoverable DNA. In five teeth that generated informative STR profiles, we assume that not all the tooth root cementum was decalcified using a non-destructive extraction method, and after grinding the whole tooth, some cementum tissue remained, contributing to successful genetic typing. In this study, the importance of tooth root cementum in archaeological samples is emphasized while exploring the forensic relevance of various tooth tissues. It highlights the notably poorer DNA preservation in dental pulp and dentin compared to cementum in the context of aged teeth. tooth root cementum archaeological samples DNA preservation and teeth tooth tissue variation STR profiling cement zobne korenine arheološki vzorci ohranjanje DNK v zobeh variabilnost zobnega tkiva STR profiliranje true false true Angleški jezik Slovenski jezik Članek v reviji 2026-02-17 07:41:34 2026-02-17 07:41:40 2026-07-10 10:39:24 0000-00-00 00:00:00 2026 0 0 8 str. art. 112882 Vol. 382 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 61:34 1872-6283 10.1016/j.forsciint.2026.112882 267937027 RAZ_Brezar_Hana_2026.pdf RAZ_Brezar_Hana_2026.pdf 1 6E7835B1288D4D30C7391337219A2CF9 46587d77d1730bfc34c47c0dd390f6d03258ca1c0b78e3feb0e5d8ae7eb51396 e2d47d8b-0bcb-11f1-a1ba-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=228143 https://www.sciencedirect.com/science/article/pii/S0379073826000691 1 7d9a3231-0bcb-11f1-a1ba-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=228141 Medicinska fakulteta Filozofska fakulteta 0 0 0