20.500.12556/RUL-179102 The effect of bisphenol PH and seven other bisphenol A alternatives on human haemostasis in vitro Recognition of bisphenol A’s toxicity has meant substitution by new analogues, which are poorly investigated. We determined the impact of bisphenol A and 8 of its analogues on platelet aggregation, coagulation, and their toxicity to erythrocytes. Erythrocyte lysis assays revealed bisphenol toxicity towards both human and rat erythrocytes with high TC$_{50s}$> 100 μM. Bisphenol A and bisphenol PH acted as antiplatelet compounds. Bisphenol PH was particularly potent (IC$_{50}$ of 0.42 ± 0.14 μM; 0.16 ± 0.05 μg/ml) in arachidonic acid-based platelet aggregation. Mechanistically, bisphenol PH blocked cyclooxygenase 1, in a similar manner to the antiplatelet drug acetylsalicylic acid. In terms of the coagulation cascade, only weak effects were found for some of the selected compounds, and the tested bisphenols did not impact coagulation or demonstrate erythrocytic toxicity at biologically achievable concentrations. Contrarily, the negative impact of bisphenol PH on platelets, with a possible subsequent risk of bleeding, might have biological relevance. bisphenol cytotoxicity haemostasis cyclooxygenase platelet bisfenol citotoksičnost hemostaza ciklooksigenaza trombociti toksikologija endokrini vidik hemostaza strjevanje krvi antikoagulacijsko zdravljenje terapija z zdravili true false true Angleški jezik Slovenski jezik Članek v reviji 2026-02-05 09:47:31 2026-02-05 09:47:35 2026-02-06 04:53:00 0000-00-00 00:00:00 2026 0 0 1-9 str. [art. no.] ǂ104897 Vol. 121 Jan. 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615.9:612.43:620.266.1 1872-7077 10.1016/j.etap.2025.104897 260908547 1-s2.0-S1382668925002728-main.pdf 1-s2.0-S1382668925002728-main.pdf 1 B1E9F7D189AE79654DF2552B11D3A7B1 5084953d236c11d86546d7874daaa1d4ecc268c26f9d795f0aaca3b5851c8959 36dd97b6-0270-11f1-a1ba-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=227243 https://www.sciencedirect.com/science/article/pii/S1382668925002728 1 3aa610ee-026f-11f1-a1ba-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=227241 Fakulteta za farmacijo 0 0 0