20.500.12556/RUL-178896 Hybrid electrospun fibers for rapid delivery of Lactobacillus paragasseri K7 and lactoferrin as live biotherapeutics and postbiotics This study explores the development of electrospun nanofibrous materials as delivery systems for the probiotic strain Lactobacillus paragasseri K7 (LK7) and the bioactive glycoprotein lactoferrin (LF) with applications targeting vaginal health. Electrospinning was used to encapsulate LK7 and LF into poly(ethylene oxide) (PEO)-based nanofibers supported on polypropylene fabric. Three formulations─PEO/LF, PEO/lactobacilli (LB) (with LK7), and PEO/LF/LB─were characterized for their physicochemical properties, fiber morphology (SEM), chemical composition (FTIR, XPS), and antioxidant activity (2,2′-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) (ABTS) assay). SEM analysis confirmed successful nanofiber formation, though LK7 remained on the fiber surface due to its size. FTIR and XPS analyses verified the incorporation of functional groups and elements associated with LF and LK7. The antioxidant assays showed that both LF and LK7 exhibited strong radical scavenging activity in formulations and it decreased slightly after electrospinning. Among the electrospun samples, the PEO/LF/LB formulation demonstrated the highest antioxidant potential. The viability and release studies revealed that 0.38–0.45% of LK7 survived during the electrospinning process and that the bacterial cells were released rapidly within 1 min of PBS exposure. Storage at 8 or 20 °C under 65% humidity reduced the viability (cfu) further, likely due to a transition to a viable but nonculturable (VBNC) state. Despite the low survival rates, the immediate release profile and antimicrobial potential of the materials support their suitability for short-term therapeutic applications such as vaginal tampons or wound dressings. This study highlights the potential of nozzle-free electrospinning for developing delivery systems for live biotherapeutics and postbiotics and suggests future work to optimize viability or expand into postbiotic applications. bacteria dietary supplements electrospinnin fibers nanofibers mikrobiologija bioterapevtiki postbiotiki Lactobacillus paragasseri laktoferin dostavni sistem nanovlakna vagina true false false ACS Angleški jezik Slovenski jezik Članek v reviji 2026-02-01 05:01:52 2026-02-03 14:18:16 2026-02-04 04:36:56 0000-00-00 00:00:00 2026 0 0 Str. 5131–5143 issue 4 Vol. 11 23 Jan. 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2026-01-23 66.02 2470-1343 10.1021/acsomega.5c07130 266096899 RAZ_Simonic_Marjana_2026.pdf RAZ_Simonic_Marjana_2026.pdf 1 67F84E20166E6E49A6B977F4D386D21A 2b84a2573348af30c697bb321f7febd19cdca768c4373b8afd285ea24d556e21 cc09afb2-0102-11f1-a1ba-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=227118 https://pubs.acs.org/doi/10.1021/acsomega.5c07130 1 bb046084-0102-11f1-a1ba-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=227116 Biotehniška fakulteta 0 0 0