20.500.12556/RUL-178383
Intramolecular sensitization and structure of a Tb$^{3+}$/2-hydroxyquinoline conjugate in the paraoxonase 1 active site
Paraoxonase 1 (PON1) is a Ca$^{2+}$-dependent enzyme involved in oxidative stress processes and is widely studied for its protective roles in various diseases. Intermolecular sensitization of lanthanide ions was implemented by replacing Ca$^{2+}$ ions from the recombinant PON1 (rePON1) catalytic site in the presence of 2-hydroxyquinoline (2HQ) as an external antenna. Although the replacement of Ca$^{2+}$ ions with lanthanide ions indicates weaker binding affinity for the coordination of 2HQ in the protein milieu of the rePON1 active site, it results in the formation of a highly emissive supramolecular complex in the case of Tb$^{3+}$ ions. The architecture of the ternary rePON1 : Tb$^{3+}$ : 2HQ conjugate, which allows efficient terbium sensitization and its specific long-wavelength metal phosphorescence emission, was resolved by X-ray crystallography. These findings could establish a non-catalytic quantification strategy for PON1 and provide additional structural insights into lanthanide substitution in this Ca$^{2+}$-dependent enzyme.
intramolecular sensitization
structure of a conjugate
paraoxonase 1
intramolekularna senzibilizacija
struktura konjugata
paraoksonaza 1
true
false
true
Angleški jezik
Slovenski jezik
Članek v reviji
2026-01-26 12:08:54
2026-01-26 12:08:58
2026-01-27 04:23:10
0000-00-00 00:00:00
2025
0
0
Str. 12471-12481
iss. 33
Vol. 54
2025
0000-00-00
Zaloznikova
Objavljeno
NiDoloceno
0000-00-00
0000-00-00
0000-00-00
577
1477-9234
10.1039/d5dt01484k
249471491
d5dt01484k.pdf
d5dt01484k.pdf
1
B9CAC6721693F6907B732757974D0C8D
6a4c1b82b606225bd5d120d9d27d766e6356984f30b97afc324d857f51c5831e
ab83d175-faa7-11f0-a1ba-0050569b8976
https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=226178
https://pubs.rsc.org/en/content/articlelanding/2025/dt/d5dt01484k
1
676690d8-faa7-11f0-a1ba-0050569b8976
https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=226177
Medicinska fakulteta
Biotehniška fakulteta
Fakulteta za kemijo in kemijsko tehnologijo
0
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0