20.500.12556/RUL-178195 Passage of the channel-forming agent nystatin through ergosterol-containing lipid membranes The passage of nystatin through the ergosterol-containing phospholipid bilayer was studied on monovesicular and multivesicular giant unilamellar vesicles (GUVs and MVVs). Phase-contrast optical microscopy was used to examine vesicles composed of a palmitoyl-oleoyl-phosphatidyl-choline (POPC) bilayer with either 15 or 45 mol% ergosterol. Three types of vesicles were analyzed: (i) GUVs, (ii) outer vesicles (outGUVs) of MVVs, and (iii) inner vesicles (inGUVs) of MVVs. The times of their ruptures were determined after their exposure to nystatin at concentrations of 250 and 500 μ M. At both concentrations, the times that the inGUVs spent in the nystatin solution after the rupture of the corresponding outGUVs were significantly shorter than the rupture times of individual GUVs of the similar size. These differences in rupture times demonstrate that the ergosterol-containing POPC membrane is permeable to nystatin. phospholipid vesicle ergosterol nystatin transmembrane passage fosfolipidni vezikel ergosterol nistatin transmembranski prehod true false true Angleški jezik Slovenski jezik Članek v reviji 2026-01-21 09:31:52 2026-01-21 09:31:55 2026-01-22 04:36:25 0000-00-00 00:00:00 2025 0 0 Str. 415-427 no. 5 Vol. 258 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-01-01 577 1432-1424 10.1007/s00232-025-00354-3 243700995 Tinev-2025-Passage-of-the-channel-forming-agen.pdf Tinev-2025-Passage-of-the-channel-forming-agen.pdf 1 1A670106AACC463C9041B65663C6A45F 9e012834c38809e18fdf402cd0f0640062b80e7e0a86762590d1371d1b94e74c bb58571e-f6a3-11f0-bf92-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=225793 https://link.springer.com/article/10.1007/s00232-025-00354-3#citeas 1 96b1fc6e-f6a3-11f0-bf92-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=225792 Zdravstvena fakulteta Medicinska fakulteta 0 0 0