20.500.12556/RUL-178104 Differential effects of amphetamine on ultrasonic vocalizations and locomotor activity in a rat model of endogenous depression The Wistar-Kyoto rat strain (WKY) is a model for treatment-resistant depression, exhibiting behaviors indicative of anhedonia. Although anhedonia is measured using the sucrose preference test (SPT), 50-kHz ultrasonic vocalizations (USVs) hold promise as a complementary assessment tool. This study aimed to detect whether 50-kHz USVs differ in the WKY rats compared to control Wistar (W) rats. First, WKY and W rats were compared for their basal 50-kHz calls. Then, rats were repeatedly treated with four administrations of either amphetamine (AMPH) or morphine (MORPH), and 50-kHz calls were recorded. In addition, the drug-evoked locomotor activity, sucrose consumption in the SPT, and the time spent in the drug- vs. saline-paired compartment in the conditioned place preference (CPP) test were collected. WKY rats emitted fewer basal calls and fewer calls following the first and the fourth AMPH administration. Contrary to controls, AMPH did not affect the number of calls in WKY rats following the first administration. WKY also displayed reduced AMPH-induced locomotor activity and sucrose consumption in the SPT. MORPH did not affect USVs or locomotion in either strain. The results highlight the benefits of 50-kHz calls as a complementary behavioral marker for assessing anhedonia in preclinical models of depression. Wistar-Kyoto rat ultrasonic vocalizations anhedonia amphetamine morphine podgana Wistar-Kyoto ultrazvočne vokalizacije anhedonia amfetamin morfin true false true Angleški jezik Slovenski jezik Članek v reviji 2026-01-19 09:46:44 2026-01-19 09:46:48 2026-04-22 11:45:39 0000-00-00 00:00:00 2025 0 0 17 str. art. 43328 Vol. 15 Dec. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-092 2045-2322 10.1038/s41598-025-27298-x 264750339 RAZ_Banjac_Anamarija_2025.pdf RAZ_Banjac_Anamarija_2025.pdf 1 7DC1D38586143B61EBE63741BEF522C9 d897819fcfab84ee0b9ec52ab9c675b21b2a83f6ef4833b5f8d2f193edb3e501 bab03543-f514-11f0-bf92-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=225651 https://www.nature.com/articles/s41598-025-27298-x 1 571060da-f513-11f0-bf92-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=225649 Medicinska fakulteta 0 0 0