20.500.12556/RUL-178057 Population structure of genotypes and genome-wide association studies of cannabinoids and terpenes synthesis in hemp (Cannabis sativa L.) Hemp (Cannabis sativa L.) is one of the oldest cultivated plants in the world. It is a wind-pollinated and heterozygous species, and diverse phenotypes can occur within population varieties. In our study, three different hemp varieties—(‘Carmagnola Selected’ (CS), ‘Tiborszallasi’ (TS) and ‘Finola selection’ (FS))—were grown. Based on visual characteristics, two, five and four phenotypes were identified within CS, TS and FS, respectively. According to Cannabis sativa L. transcriptome data from the Sequence Read Archive (SRA), 4631 single-nucleotide polymorphism (SNP) positions were identified to develop capture probes. DNA was isolated from 171 plants representing selected phenotypes of three cultivars. Next-generation sequencing (NGS) libraries were constructed and hybridized with capture probes for target enrichment. The population structure of the samples was analyzed using SNP data for each genotype. Based on genotype profiles, CS formed a single cluster, while TS and FS were each grouped into two clusters, with phenotypes randomly distributed among them. The GWAS results were visualized using Manhattan plots. Fourteen significant SNPs surpassing the false discovery rate (FDR) of 0.01 were identified for delta-9-tetrahydrocannabinol (delta-9-THC). For cannabigerol (CBG), 12 significant SNPs were detected, and for myrcene, one SNP exceeded the 0.01 FDR threshold. However, plausible genes located 1000 bp to the left and right of the SNP position were identified for all significant SNPs. Cannabis sativa genotype GWAS hemp population structure SNP konoplja populacijska struktura genotipi kanabinoidi terpeni true false true Angleški jezik Slovenski jezik Članek v reviji 2026-01-16 15:37:28 2026-01-16 15:37:35 2026-01-17 04:38:20 0000-00-00 00:00:00 2026 0 0 20 str. iss. 2, art. 202 Vol. 15 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 633 2223-7747 10.3390/plants15020202 265063427 plants-15-00202-v2.pdf plants-15-00202-v2.pdf 1 8FE860A96C5D25E0A33ED58C9A3D3D70 36a125ec246dc3b727845a74487d7ccb696f547d126a02406862312e998ddc85 0636764e-f2e9-11f0-bf92-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=225587 https://www.mdpi.com/2223-7747/15/2/202 1 dc7c48a8-f2e8-11f0-bf92-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=225585 Biotehniška fakulteta 0 0 0