20.500.12556/RUL-178002 First case report of Shewanella indica isolated from a hospitalized patient in Serbia Over the past decade, Shewanella spp. have been increasingly recognized as opportunistic pathogens, particularly in patients with malignancies, neutropenia, severe heart failure, renal insufficiency, and hepatobiliary diseases. Shewanella indica is a rarely reported species within the Shewanella genus, and its role in human infection remains poorly documented. In this study, we report the first case of S. indica isolated from the stool of a critically ill 72-year-old man in Serbia with multiple pre-existing comorbidities and co-infections. Initial identification using the VITEK 2 system misclassified the strain as Shewanella algae, while species-level identification by the matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) was inconclusive. However, whole-genome sequencing (WGS) definitively identified the isolate as S. indica and revealed a multidrug-resistant profile together with numerous virulence-associated genes. Minimum inhibitory concentrations (MICs) were determined for 32 antibiotics, although interpretation was constrained by the lack of species-specific breakpoints. This case highlights the diagnostic challenges in differentiating Shewanella indica, discusses its possible clinical roles, and underscores the value of genomic tools for accurate identification. It also reinforces the importance of recognizing rare pathogens in complex clinical scenarios. Shewanella indica Zn4(μ4-O) entity genome sequencing species Identification and antimicrobial susceptibility Shewanella indica klinični primer sekvenciranje genoma identifikacija do vrste in odpornost proti antibiotikom true false true Angleški jezik Slovenski jezik Članek v reviji 2026-01-16 04:21:45 2026-01-16 10:18:59 2026-01-17 04:38:18 0000-00-00 00:00:00 2026 0 0 8 str. Vol. 12, art. 1715579 2026 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 579:616 2296-858X 10.3389/fmed.2025.1715579 264321539 First.pdf First.pdf 1 5BEFBC799A9705DFE9C4DE08D6A9DF9D 3901f301386b50f93915eff75244a3bfee4bbbaa308a101e4d9a10dba15aea87 d993923e-f2bd-11f0-bf92-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=225524 https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2025.1715579/full 1 776c7327-f28a-11f0-bf92-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=225486 Veterinarska fakulteta Fakulteta za farmacijo 0 0 0