20.500.12556/RUL-178001 Integrated light and electron microscopy workflow for morphological, molecular and ultrastructural analysis of spheroids research data underlying the article The dataset presented here supports an integrated light and electron microscopy workflow for comprehensive morphological, molecular, and ultrastructural characterization of three-dimensional (3D) spheroid models. The data accompany a submitted data paper and are discussed in a submitted original research article, with the aim of enabling standardized and reproducible multi-scale imaging of spheroids in research in vitro. The associated research article presents optimized workflows for light and electron microscopy analysis of spheroids derived from the human noncancerous urothelial cell line SV-HUC-1 and the malignant urothelial cancer cell line T24. The study describes protocols for histological staining and immunofluorescence on cryosectioned and paraffin-embedded spheroids, as well as whole-mount immunofluorescence combined with optical clearing and confocal microscopy for three-dimensional protein localization. Scanning and transmission electron microscopy further provide high-resolution insights into spheroid surface morphology and internal ultrastructure. Together, these complementary approaches establish a standardized framework for multi-scale spheroid characterization applicable across diverse in vitro models. bladder cancer light microscopy and electron microscopy protocols sample preparation spheroids urothelial cells rak sečnega mehurja svetlobna in elektronska mikroskopija protokoli priprava vzorcev sferoidi urotelijske celice true false false Angleški jezik Slovenski jezik Research data underlying the article were collected at Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia. 2024-2025 Neznano 2026-01-15 15:46:04 2026-01-15 15:46:09 2026-01-17 04:38:18 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 README.docx README.docx README 1 300A438379C07C4A4BDC0E2A3F1F8620 dd0af73fe62c4d02ad918a870cd6598cc90d8afb8880c27dd3e752d5f4a4c111 c0e1334c-f2d5-11f0-bf92-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=225569 Raw_images_Microscopy_models_for_3D.zip Raw_images_Microscopy_models_for_3D.zip Raw images Microscopy models for 3D 1 7BAEB41AE705AA2018C61772C62E478E 68aeeb541b2f0d84230975d3a4a9e93cd1e2eaf65dfccef046d936ffe6b94e70 187d3985-f2d5-11f0-bf92-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=225565 Medicinska fakulteta Meritve in testi 0 0 0