20.500.12556/RUL-177944 Direct and indirect effects of microplastics from agricultural mulch films on terrestrial isopods Porcellionides pruinosus (Crustacea, Isopoda) a comparative exposure study Microplastic (MPs) contamination in terrestrial ecosystems is an emerging concern, with indirect impacts on soil biota often exceeding direct toxicity. This study examined direct and indirect effects of MPs from agricultural mulch films—low-density polyethylene (LDPE), polybutylene adipate terephthalate (PBAT) and starch bioplastics (starch-based)—on the soil isopod Porcellionides pruinosus. We conducted comparative feeding and mesocosm experiments under environmentally realistic conditions. We analysed toxicological endpoints like survival and weight change, and stress-related markers like total haemocyte count, differential haemocyte count, phenoloxidase PO-like activity, enzymatic assays of acetylcholinesterase, glutathione S-transferase, and electron transport system activity. No acute toxicity was observed; however, soil exposure had a more pronounced effect on P. pruinosus, confirming that indirect impacts via altered soil conditions are stronger than direct dietary exposure. Notably, only bio-based MPs triggered changes in cellular and biochemical stress parameters, while fossil-based MPs had no observable effect. The stress response of P. pruinosus in mesocosm experiments aligned with shifts in microbial respiration, suggesting that soil-mediated effects play a dominant role in organismal stress. These findings underscore the importance of considering indirect pathways in MP toxicity assessments and highlight the suitability of terrestrial isopods for integrating diverse toxicological endpoints and stress markers in both laboratory and mesocosm settings. comparative study microplastics soil invertebrates stress related markers toxicity endpoints mikroplastika talni nevretenčarji kmetijske folije za mulčenje true false true Angleški jezik Slovenski jezik Članek v reviji 2026-01-14 11:22:54 2026-01-14 11:23:02 2026-01-15 04:21:35 0000-00-00 00:00:00 2026 0 0 12 str. art. 119741 Vol. 309 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 502.1 0147-6513 10.1016/j.ecoenv.2026.119741 264653059 1-s2.0-S0147651326000709-main.pdf 1-s2.0-S0147651326000709-main.pdf 1 5C1A77E9BF2FCB2D11022C4A2557FAF6 5c24628f1e6bdd121556cc5ab8fadab6f3f070cee05874e6bebb44b06538dd7d b379bb4d-f133-11f0-bf92-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=225389 https://www.sciencedirect.com/science/article/pii/S0147651326000709 1 f8e0ec46-f132-11f0-bf92-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=225388 Biotehniška fakulteta 0 0 0