20.500.12556/RUL-177614 Učinek gnojenja in režima košnje na arbuskularno mikorizo na srednje vlažnem polnaravnem travinju Effect of fertiliser application and cutting regime on arbuscular mycorrhiza in mesic semi-natural grassland Arbuskularne mikorizne glive kolonizirajo korenine večine vaskularnih rastlin in v simbiotskem odnosu povečujejo njihovo zmogljivost pridobivanja hranil in vode iz tal. Imajo pomembno ekosistemsko vlogo zato je njihovo ohranjanje v tleh bistvenega pomena. Talni mikroorganizmi so zelo občutljivi na kmetijske prakse. Na travinju na simbiozo negativno vplivajo intenzivne prakse, kot so sejanje monokultur posevkov, prekomerna paša, prekomerna košnja in prekomerno gnojenje. V nalogi smo bili osredotočeni na vpliv gnojenja in košnje na pojavnost in intenziteto arbuskularne mikorize na vlažnem polnaravnem travinju. Vpliv obeh dejavnikov se na Oddelku za agronomijo, Biotehniške fakultete Univerze v Ljubljani, proučuje na dolgoletnem poskusu Travistor. V naši raziskavi smo od sredine maja do sredine junija 2023 zbrali skupno 120 vzorcev izvrtkov tal s koreninami, po 3 vzorce iz vsake od 4 ploskvic na obravnavanje. V desetih obravnavanjih je bila dvokosna raba, ki vključuje dve košnji in imitacijo jesenske paše ali štirikosna raba kombinirana z različnimi odmerki mineralnih gnojil in/ali gnojevke. Iz izvrtkov smo ekstrahirali korenine, jih očistili ter po presvetljevanju obarvali. Pod mikroskopom smo ocenjevali prisotnost arbuskularne mikorize in prisotnost arbuskulov in v vseh vzorcih opazili značilne strukture arbuskularnih mikoriznih gliv. Ugotovili smo, da imajo statistično značilno višjo intenziteto mikorize in višjo intenziteto mikorize v koloniziranih delih korenine obravnavanja z dvokosno rabo v primerjavi z obravnavanji s štirikosno rabo. Rezultati niso pokazali statistično značilnega vpliva postopkov gnojenja na proučevane parametre. Arbuscular mycorrhizal fungi colonize the roots of most vascular plants. In a symbiotic relationship they enable them to obtain nutrients and water from the soil. They play an important ecosystem role, so their preservation in soil is essential. Soil microorganisms are very sensitive to agricultural practices. In grassland, symbiosis is negatively affected by intensive practices such as sowing monoculture crops, overgrazing, overmowing and overfertilization. In the thesis, we focused on the impact of fertilization and mowing on the occurrence and intensity of arbuscular mycorrhiza in moist semi-natural grassland. The impact of both factors was studied at the Department of Agronomy, Biotechnical Faculty, University of Ljubljana, in the long term Travistor experiment. In our research, we collected a total of 120 samples of soil cores with roots from mid-May to mid-June 2023, 3 samples from each of 4 replicates per treatment. In ten treatments, 2-cut regime, which includes two mowings and imitation of autumn grazing or 4-cut regime was combined with different doses of mineral fertilizers and/or slurry. Roots were extracted from the samples, cleaned and stained for chitin after transillumination. Presence of arbuscular mycorrhiza and presence of arbuscules were assessed under a microscope. Characteristic structures of arbuscular mycorrhizal fungi were observed in all samples. We found out that the treatments that were mowed twice had statistically significant higher mycorrhizal intensity and a higher mycorrhizal intensity in the colonized parts of the roots compared to the treatments that were mowed four times. Results did not show a statistically significant effect of fertilization regimes on studied parameters. arbuskularno mikorizne glive arbuskuli simbioza gnojenje košnja travinje arbuscular mycorrhizal fungi arbuscules symbiosis fertilization mowing grassland true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-12-26 07:15:55 2025-12-26 07:16:04 2026-01-12 12:45:53 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 277690 264333571 55653.pdf 55653.pdf 1 177E4C33CD71957CABE36A5C89DBBAFB 4a0173f237e1f9e2a0eb037555092af669dbd38123989f891256697c0a392827 e624b40f-e221-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=224726 Biotehniška fakulteta 0 0 0