20.500.12556/RUL-177236 Biochemical alternations in leaves of grapevine (Vitis vinifera L.) under virus-induced stress Biokemične spremembe v listih žlahtne vinske trte (Vitis vinifera L.) pod stresom zaradi virusa Grapevine leafroll disease (GLD) is one of the most prevalent and economically significant viral diseases affecting grapevines. This study aimed to detect GLRaV presence in grapevine cultivars from Azerbaijan and examine associated stress-related metabolic alterations. During the summer of 2023, a total of forty-eight symptomatic grapevine leaf samples were collected from vineyards in Salyan and Jalilabad regions. Visual symptoms included leaf rolling, interveinal reddening, mosaic patterns, and yield decline. Virus detection was performed using serological techniques (AgriStrip and DAS-ELISA) and molecular testing via RT-PCR. Results showed that 13% of samples were infected with GLRaV-2 and 23% with GLRaV-3. Biochemical profiling revealed increased malondialdehyde (MDA) accumulation, indicating elevated lipid peroxidation and oxidative stress in infected vines. Furthermore, significantly higher levels of total phenolic compounds, tocopherols, and soluble proteins were observed, suggesting enhanced non-enzymatic antioxidant responses. The activities of key antioxidant enzymes—superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX)—were markedly upregulated in virus-infected samples compared to healthy controls. These enzymatic changes highlight the grapevine’s biochemical defense response to viral stress. Overall, the study demonstrates that GLRaV infection triggers both enzymatic and non-enzymatic antioxidant systems, which may serve as potential biomarkers for virus tolerance in grapevines. ascorbate peroxidase (APX) catalase (CAT) grapevine leafroll disease lipid peroxidation superoxide dismutase (SOD) tocopherols askorbat peroksidaza (APX) katalaza (CAT) bolezen zvijanja listov vinske trte lipidna peroksidacija superoksid dismutaza (SOD) tokoferoli true false true Angleški jezik Slovenski jezik Članek v reviji 2025-12-18 09:29:47 2025-12-18 09:29:53 2025-12-29 03:55:38 0000-00-00 00:00:00 2025 0 0 10 str. no. 4 Vol. 121 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 63 1854-1941 10.14720/aas.2025.121.4.23971 261980675 SULTANOVA+et+al.+(D).pdf SULTANOVA+et+al.+(D).pdf 1 5459558D74ABA2DDB67D2BEA32890022 90ac3d01abf746dc84692c676a670d0680b21a5b9b3c4a36756bc0cd215dff20 5fab69fc-dbeb-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=224156 https://journals.uni-lj.si/aas/article/view/23971/19769 1 49b9f1c7-dbeb-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=224155 Biotehniška fakulteta Acta agriculturae Slovenica 0 0 0